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Using microfluidics and live cell reporters to dissect the dynamics of TGF-β signaling in mouse Embryonic Stem Cells

Abstract : The TGF-β pathway is known to behave as a classical morphogen, meaning that it can dictate cell fate decisions in a dose dependent manner. Recent observations however showed that in addition to the absolute value of morphogen concentration, cells could also extract information from its temporal variations. In the present article we describe how to use automated microfluidics cell culture to stimulate cells with precisely defined temporal profiles of morphogens and how to engineer mouse embryonic stem cells with fluorescent reporters of pathway activity to record in real time their response to the applied stimulations. The combination of automated cell culture and of live cell reporter provides a complete toolbox to study how cells encode the information carried by time-varying TGF-β signals.
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https://hal.archives-ouvertes.fr/hal-03437837
Contributor : Benoit Sorre Connect in order to contact the contributor
Submitted on : Saturday, November 20, 2021 - 5:31:25 PM
Last modification on : Friday, August 5, 2022 - 11:58:02 AM
Long-term archiving on: : Tuesday, February 22, 2022 - 10:06:20 AM

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  • HAL Id : hal-03437837, version 1

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Fabien Furfaro, Carine Vias, Benoit Sorre. Using microfluidics and live cell reporters to dissect the dynamics of TGF-β signaling in mouse Embryonic Stem Cells. Methods in Molecular Biology, Humana Press/Springer Imprint, In press. ⟨hal-03437837⟩

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