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Discovery of Escherichia coli methionyl-tRNA synthetase mutants for efficient labeling of proteins with azidonorleucine in vivo.

Abstract : Incorporation of noncanonical amino acids into cellular proteins often requires engineering new aminoacyl-tRNA synthetase activity into the cell. A screening strategy that relies on cell-surface display of reactive amino acid side-chains was used to identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants that allow efficient incorporation of the methionine (Met) analog azidonorleucine (Anl). We demonstrate that the extent of cell-surface labeling in vivo is a good indicator of the rate of Anl activation by the MetRS variant harbored by the cell. By screening at low Anl concentrations in Met-supplemented media, MetRS variants with improved activities toward Anl and better discrimination against Met were identified.
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https://hal-polytechnique.archives-ouvertes.fr/hal-00502034
Contributor : Yves Mechulam Connect in order to contact the contributor
Submitted on : Tuesday, July 13, 2010 - 10:12:04 AM
Last modification on : Wednesday, July 29, 2020 - 4:10:05 PM

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I Caglar Tanrikulu, Emmanuelle Schmitt, Yves Mechulam, William A Goddard, David A Tirrell. Discovery of Escherichia coli methionyl-tRNA synthetase mutants for efficient labeling of proteins with azidonorleucine in vivo.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2009, 106 (36), pp.15285-90. ⟨10.1073/pnas.0905735106⟩. ⟨hal-00502034⟩

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