Multiphoton microscopy at an excitation wavelength of 1.26 µm

Karsten Plamann (1) , Gilbert Boyer (1) , Denis Chemla (2)

1 LOA - Laboratoire d'optique appliquée
2 Hôpital Bicêtre

We present a multiphoton microscope using a Cr4 + :forsterite femtosecond laser with an emission wavelength of 1260 nm for the excitation of the multiphoton processes. This wavelength is well adapted to the "optical window" in biological tissues and permits to reach higher imaging depths than systems using more conventional titanium:sapphire laser sources. The paper describes the experimental set-up and reports on first results on human cornea and skin samples.

Mots clés: refractive surgery; dermatology; Multi-photon microscopy; Cr4+:forsterite laser; imaging depth; optical window in tissues

Domaines: Optique [physics.optics]

Karsten Plamann, Gilbert Boyer, Denis Chemla. Multiphoton microscopy at an excitation wavelength of 1.26 µm. Proceedings of SPIE, the International Society for Optical Engineering, 2003, 5139, pp.42. ⟨10.1117/12.500487⟩. ⟨hal-00527974⟩