Modular model-based design for heterologous bioproduction in bacteria.

T.E. Landrain (1) , J. Carrera (1, 2, 3) , B. Kirov (1) , G. Rodrigo (2, 1) , A. Jaramillo (1, 4)

1 Programme d'Épigénomique
2 Instituto de Biología Molecular y Celular de Plantas
3 Instituto ITACA
4 BIOC - Laboratoire de Biochimie de l'Ecole polytechnique

We review the current status of expression of heterologous systems for bioenergy and bioproduction in bacteria using a model-based approach. As an aim for synthetic biology, it requires mathematical models of genetic modules that could be characterized independently of their context. This fastens the design of metabolic circuits using a combinatorial design approach, where given pathways could be optimized for maximal bioproduction, while being nontoxic for the chassis. We show how recent characterization of genetic parts, such as promoters, RBS or sRNAs could be used to fine-tune the expression of individual genes to achieve that goal. We also present lists of enzymes that are used for bioproduction, enlarging such set of biological parts.

Domains: Biochemistry, Molecular Biology

T.E. Landrain, J. Carrera, B. Kirov, G. Rodrigo, A. Jaramillo. Modular model-based design for heterologous bioproduction in bacteria.. Current Opinion in Biotechnology, 2009, 20 (3), pp.272-9. ⟨10.1016/j.copbio.2009.06.003⟩. ⟨hal-00766033⟩