Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Widespread distribution of cell defense against D-aminoacyl-tRNAs.

Abstract : Several l-aminoacyl-tRNA synthetases can transfer a d-amino acid onto their cognate tRNA(s). This harmful reaction is counteracted by the enzyme d-aminoacyl-tRNA deacylase. Two distinct deacylases were already identified in bacteria (DTD1) and in archaea (DTD2), respectively. Evidence was given that DTD1 homologs also exist in nearly all eukaryotes, whereas DTD2 homologs occur in plants. On the other hand, several bacteria, including most cyanobacteria, lack genes encoding a DTD1 homolog. Here we show that Synechocystis sp. PCC6803 produces a third type of deacylase (DTD3). Inactivation of the corresponding gene (dtd3) renders the growth of Synechocystis sp. hypersensitive to the presence of d-tyrosine. Based on the available genomes, DTD3-like proteins are predicted to occur in all cyanobacteria. Moreover, one or several dtd3-like genes can be recognized in all cellular types, arguing in favor of the nearubiquity of an enzymatic function involved in the defense of translational systems against invasion by d-amino acids.
Document type :
Journal articles
Complete list of metadata
Contributor : Denis Roura Connect in order to contact the contributor
Submitted on : Monday, December 17, 2012 - 2:32:30 PM
Last modification on : Wednesday, December 15, 2021 - 10:00:38 AM

Links full text




Sandra Wydau, G. van Der Rest, Caroline Aubard, Pierre Plateau, Sylvain Blanquet. Widespread distribution of cell defense against D-aminoacyl-tRNAs.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2009, 284 (21), pp.14096-104. ⟨10.1074/jbc.M808173200⟩. ⟨hal-00766038⟩



Record views