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Journal articles
Analysis of protein sequences and protein complexes by matrix-assisted laser desorption/ionization mass spectrometry.
Abstract : In the context of proteome analysis, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) can fulfil the two tasks of primary structure verification and protein identification. As an illustration of the first of these tasks, the sequence of Eschericha coli isoleucyl-tRNA synthetase, a protein with 15 reported sequence conflicts, has been established by means of MALDI mass mapping. The identification of mitochondrial proteins participating in a yeast supramolecular complex exhibiting NADH dehydrogenase activity highlights the performances of MALDI-MS for the second task. The spectral suppression phenomenon occurring for complex peptide mixtures analysed by MALDI is discussed, as well as the role of post-source decay analysis for confident protein identification.
https://hal-polytechnique.archives-ouvertes.fr/hal-00771534
Contributor : Denis Roura Connect in order to contact the contributor
Submitted on : Tuesday, January 8, 2013 - 7:18:48 PM
Last modification on : Wednesday, July 29, 2020 - 4:10:05 PM
Contributor : Denis Roura Connect in order to contact the contributor
Submitted on : Tuesday, January 8, 2013 - 7:18:48 PM
Last modification on : Wednesday, July 29, 2020 - 4:10:05 PM
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