Measurement of circular dichroism dynamics in a nanosecond temperature-jump experiment

Mai-Thu Khuc 1 Lucille Mendonça 1 S. Sharma 2 M. Volk 2 Xavier Solinas 1 François Hache 1, *
* Corresponding author
1 LOB - Laboratoire d'optique et biosciences
2 University of Liverpool
Abstract : The use of a fast temperature jump (T-jump) is a very powerful experiment aiming at studying protein denaturation dynamics. However, probing the secondary structure is a difficult challenge and rarely yields quantitative values. We present the technical implementation of far-UV circular dichroism in a nanosecond T-jump experiment and show that this experiment allows us to follow quantitatively the change in the helical fraction of a poly(glutamic acid) peptide during its thermal denaturation with 12 ns time resolution. Cop. 2011 American Institute of Physics.
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Mai-Thu Khuc, Lucille Mendonça, S. Sharma, M. Volk, Xavier Solinas, et al.. Measurement of circular dichroism dynamics in a nanosecond temperature-jump experiment. Review of Scientific Instruments, American Institute of Physics, 2011, 82 (5), ⟨10.1063/1.3592331⟩. ⟨hal-00803784⟩

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