Measurement of circular dichroism dynamics in a nanosecond temperature-jump experiment
Abstract
The use of a fast temperature jump (T-jump) is a very powerful experiment aiming at studying protein denaturation dynamics. However, probing the secondary structure is a difficult challenge and rarely yields quantitative values. We present the technical implementation of far-UV circular dichroism in a nanosecond T-jump experiment and show that this experiment allows us to follow quantitatively the change in the helical fraction of a poly(glutamic acid) peptide during its thermal denaturation with 12 ns time resolution. Cop. 2011 American Institute of Physics.
Domains
Optics [physics.optics]
Origin : Publisher files allowed on an open archive
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