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Journal Articles Review of Scientific Instruments Year : 2011

Measurement of circular dichroism dynamics in a nanosecond temperature-jump experiment

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Abstract

The use of a fast temperature jump (T-jump) is a very powerful experiment aiming at studying protein denaturation dynamics. However, probing the secondary structure is a difficult challenge and rarely yields quantitative values. We present the technical implementation of far-UV circular dichroism in a nanosecond T-jump experiment and show that this experiment allows us to follow quantitatively the change in the helical fraction of a poly(glutamic acid) peptide during its thermal denaturation with 12 ns time resolution. Cop. 2011 American Institute of Physics.
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Dates and versions

hal-00803784 , version 1 (17-04-2014)

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Mai-Thu Khuc, Lucille Mendonça, S. Sharma, M. Volk, Xavier Solinas, et al.. Measurement of circular dichroism dynamics in a nanosecond temperature-jump experiment. Review of Scientific Instruments, 2011, 82 (5), ⟨10.1063/1.3592331⟩. ⟨hal-00803784⟩
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