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Measurement of circular dichroism dynamics in a nanosecond temperature-jump experiment
Abstract : The use of a fast temperature jump (T-jump) is a very powerful experiment aiming at studying protein denaturation dynamics. However, probing the secondary structure is a difficult challenge and rarely yields quantitative values. We present the technical implementation of far-UV circular dichroism in a nanosecond T-jump experiment and show that this experiment allows us to follow quantitatively the change in the helical fraction of a poly(glutamic acid) peptide during its thermal denaturation with 12 ns time resolution. Cop. 2011 American Institute of Physics.
Cited literature [21 references]
https://hal-polytechnique.archives-ouvertes.fr/hal-00803784
Contributor : Denis Roura Connect in order to contact the contributor
Submitted on : Thursday, April 17, 2014 - 8:58:42 PM
Last modification on : Thursday, November 18, 2021 - 3:42:02 PM
Long-term archiving on: : Sunday, April 2, 2017 - 6:58:06 PM
Contributor : Denis Roura Connect in order to contact the contributor
Submitted on : Thursday, April 17, 2014 - 8:58:42 PM
Last modification on : Thursday, November 18, 2021 - 3:42:02 PM
Long-term archiving on: : Sunday, April 2, 2017 - 6:58:06 PM
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RSI2011.pdf
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