Dispersion-based pulse shaping for multiplexed two-photon fluorescence microscopy

Guillaume Labroille (1) , Rajesh Pillai (1) , Xavier Solinas (1) , Caroline Boudoux (1, 2) , Nicolas Olivier (1) , Emmanuel Beaurepaire (1) , Manuel Joffre (1)

1 LOB - Laboratoire d'optique et biosciences
2 Centre d'Optique, Photonique et Laser

We demonstrate selective two-photon excited fluorescence microscopy with shaped pulses produced with a simple yet efficient scheme based on dispersive optical components. The pulse train from a broadband oscillator is split into two subtrains that are sent through different amounts of glass. Beam recombination results in pulse-shape switching at a rate of 150 MHz. Time-resolved photon counting detection then provides two simultaneous images resulting from selective two-photon excitation, as demonstrated in a live embryo. Although less versatile than programmable pulse-shaping devices, this novel arrangement significantly improves the performance of selective microscopy using broadband shaped pulses while simplifying the experimental setup. Cop. 2010 Optical Society of America.

Domains: Optics [physics.optics]; Imaging

Guillaume Labroille, Rajesh Pillai, Xavier Solinas, Caroline Boudoux, Nicolas Olivier, et al.. Dispersion-based pulse shaping for multiplexed two-photon fluorescence microscopy. Optics Letters, 2010, 35 (20), pp.3444. ⟨10.1364/OL.35.003444⟩. ⟨hal-00805068⟩