Multiplexed two-photon microscopy of dynamic biological samples with shaped broadband pulses.

Rajesh Pillai 1 Caroline Boudoux 1, 2 Guillaume Labroille 1 Nicolas Olivier 1 Israel Veilleux 1 Emmanuel Farge 2 Manuel Joffre 1 Emmanuel Beaurepaire 1, *
* Corresponding author
1 LOB - Laboratoire d'optique et biosciences
2 Ecole Polytechnique
Abstract : Coherent control can be used to selectively enhance or cancel concurrent multiphoton processes, and has been suggested as a means to achieve nonlinear microscopy of multiple signals. Here we report multiplexed two-photon imaging in vivo with fast pixel rates and micrometer resolution. We control broadband laser pulses with a shaping scheme combining diffraction on an optically-addressed spatial light modulator and a scanning mirror allowing to switch between programmable shapes at kiloHertz rates. Using coherent control of the two-photon excited fluorescence, it was possible to perform selective microscopy of GFP and endogenous fluorescence in developing Drosophila embryos. This study establishes that broadband pulse shaping is a viable means for achieving multiplexed nonlinear imaging of biological tissues.
Document type :
Journal articles
Complete list of metadatas

Cited literature [35 references]  Display  Hide  Download
https://hal-polytechnique.archives-ouvertes.fr/hal-00818488
Contributor : Denis Roura <>
Submitted on : Saturday, October 12, 2013 - 10:50:21 AM
Last modification on : Thursday, February 7, 2019 - 4:30:20 PM
Long-term archiving on : Monday, January 13, 2014 - 2:40:23 AM

File

oe-17-15-12741.pdf
Publisher files allowed on an open archive

Identifiers

Collections

X-LOB | PARISTECH | X | X-DEP-BIO

Citation

Rajesh Pillai, Caroline Boudoux, Guillaume Labroille, Nicolas Olivier, Israel Veilleux, et al.. Multiplexed two-photon microscopy of dynamic biological samples with shaped broadband pulses.. Optics Express, Optical Society of America, 2009, 17 (15), pp.12741-52. ⟨10.1364/OE.17.012741⟩. ⟨hal-00818488⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

357

Files downloads

203

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD