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Journal articles
Multiplexed two-photon microscopy of dynamic biological samples with shaped broadband pulses.
Abstract : Coherent control can be used to selectively enhance or cancel concurrent multiphoton processes, and has been suggested as a means to achieve nonlinear microscopy of multiple signals. Here we report multiplexed two-photon imaging in vivo with fast pixel rates and micrometer resolution. We control broadband laser pulses with a shaping scheme combining diffraction on an optically-addressed spatial light modulator and a scanning mirror allowing to switch between programmable shapes at kiloHertz rates. Using coherent control of the two-photon excited fluorescence, it was possible to perform selective microscopy of GFP and endogenous fluorescence in developing Drosophila embryos. This study establishes that broadband pulse shaping is a viable means for achieving multiplexed nonlinear imaging of biological tissues.
Cited literature [35 references]
https://hal-polytechnique.archives-ouvertes.fr/hal-00818488
Contributor : Denis Roura <>
Submitted on : Saturday, October 12, 2013 - 10:50:21 AM
Last modification on : Wednesday, October 14, 2020 - 4:19:12 AM
Long-term archiving on: : Monday, January 13, 2014 - 2:40:23 AM
Contributor : Denis Roura <>
Submitted on : Saturday, October 12, 2013 - 10:50:21 AM
Last modification on : Wednesday, October 14, 2020 - 4:19:12 AM
Long-term archiving on: : Monday, January 13, 2014 - 2:40:23 AM
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oe-17-15-12741.pdf
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