Endothelial nitric oxide synthase reduces nitrite anions to NO under anoxia

Clément Gautier 1 E. van Faassen 1 I. Mikula 1 P. Martasek 1 Anny Slama-Schwok 1, 2, 3, 4
1 LOB - Laboratoire d'optique et biosciences
2 Univ Utrecht, Debye Inst, Sect Interface Phys, NL-3508 TC Utrecht, Netherlands
3 Charles Univ, Sch Med 1, Dept Pediat, Prague, Czech Republic
4 Charles Univ, Sch Med 1, Ctr Appl Genom, Prague, Czech Republic
Abstract : In this work, we demonstrate that endothelial nitric oxide synthase is capable of anoxic reduction of nitrite anions to nitric oxide at physiological pH by absorption and EPR spectroscopy and electrochemical measurements. The nitrite reduction is achieved at the oxygenase domain of the protein and proceeds even in the absence of the tetrahydrobiopterin cofactor. The nitrite pathway increases by sixfold the NO production with respect to the regular arginine pathway under hypoxia, which is largely blocked. Therefore, basal levels of NO release could be sustained by anoxic nitrite reduction. The reaction suggests a new pathway for fast NO delivery under hypoxia, precisely when the vasodilating properties of nitric oxide are most needed. (c) 2006 Elsevier Inc. All rights reserved.
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Submitted on : Sunday, May 26, 2013 - 9:49:11 PM
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Clément Gautier, E. van Faassen, I. Mikula, P. Martasek, Anny Slama-Schwok. Endothelial nitric oxide synthase reduces nitrite anions to NO under anoxia. Biochemical and Biophysical Research Communications, Elsevier, 2006, 341 (3), pp.816. ⟨10.1016/j.bbrc.2006.01.031⟩. ⟨hal-00824465⟩

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