Skip to Main content Skip to Navigation
Journal articles

Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity

D.E. Hunt 1 V. Klepac-Ceraj 1, 2 S.G. Acinas 1 S. Bertilsson 1, 3 Christian Gautier 1, 4 M.F. Polz 1 
1 CEE - Department of Civil and Environmental Engineering [Cambridge]
2 Department of Microbiology and Molecular Genetics
3 Limnology [Norbyvägen]
4 LOB - Laboratoire d'optique et biosciences
Abstract : The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample. Copyright Cop. 2006, American Society for Microbiology. All Rights Reserved.
Document type :
Journal articles
Complete list of metadata
https://hal-polytechnique.archives-ouvertes.fr/hal-00824466
Contributor : Denis Roura Connect in order to contact the contributor
Submitted on : Sunday, May 26, 2013 - 9:43:39 PM
Last modification on : Saturday, February 27, 2021 - 1:48:01 PM

Links full text

pubmedcentral

Identifiers

Collections

X | CNRS | X-LOB | X-DEP-BIO

Citation

D.E. Hunt, V. Klepac-Ceraj, S.G. Acinas, S. Bertilsson, Christian Gautier, et al.. Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity. Applied and Environmental Microbiology, American Society for Microbiology, 2006, 72 (3), pp.2221. ⟨10.1128/AEM.72.3.2221-2225.2006⟩. ⟨hal-00824466⟩

Export

BibTeX TEI DC DCterms EndNote Datacite

Share

Metrics

Record views

52