Functionality of nitrated acetylcholine receptor: The two-step formation of nitrotyrosines reveals their differential role in effectors binding

Michel Négrerie 1 Jean-Louis Martin 1 H.-O. Nghiêm 2
1 LOB - Laboratoire d'optique et biosciences
2 RC - Récepteurs et Cognition
Abstract : The presence of nitrotyrosines is associated with several neurodegenerative pathologies. We evaluated the functionality of the nicotinic acetylcholine receptor possessing nitrotyrosines. The spectrum of the nitrated receptor displays an absorption band characteristic of ortho-nitrophenol. The presence of carbamylcholine in the agonist site prevented the effect of nitration by tetranitromethane in some conditions. The nitration occurred with two discrete steps and pointed out the differential involvement of tyrosines in the binding of acetylcholine and neurotoxin. We concluded that at least two residues involved in agonist binding can be nitrated, which bring similar contributions to the binding energy of the neurotransmitter. Cop. 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Document type :
Journal articles
Complete list of metadatas
https://hal-polytechnique.archives-ouvertes.fr/hal-00829232
Contributor : Denis Roura <>
Submitted on : Thursday, June 20, 2013 - 9:08:54 PM
Last modification on : Thursday, February 7, 2019 - 2:54:30 PM

Links full text

openaccess

Identifiers

Collections

X-LOB | PASTEUR | X | PARISTECH | PSL | X-DEP-BIO

Citation

Michel Négrerie, Jean-Louis Martin, H.-O. Nghiêm. Functionality of nitrated acetylcholine receptor: The two-step formation of nitrotyrosines reveals their differential role in effectors binding. FEBS Letters, Wiley, 2005, 579 (12), pp.2643. ⟨10.1016/j.febslet.2005.03.084⟩. ⟨hal-00829232⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

192

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD