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Journal articles
In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses
Abstract : The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.
https://hal-polytechnique.archives-ouvertes.fr/hal-00829234
Contributor : Denis Roura <>
Submitted on : Tuesday, June 18, 2013 - 10:00:34 PM
Last modification on : Tuesday, December 8, 2020 - 3:33:23 AM
Contributor : Denis Roura <>
Submitted on : Tuesday, June 18, 2013 - 10:00:34 PM
Last modification on : Tuesday, December 8, 2020 - 3:33:23 AM
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