In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses - Archive ouverte HAL Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2005

In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses

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Abstract

The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.

Dates and versions

hal-00829234 , version 1 (18-06-2013)

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Willy Supatto, Delphine Débarre, Bruno B. Moulia, Eric Brouzés, Jean-Louis Martin, et al.. In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102 (4), pp.1047. ⟨10.1073/pnas.0405316102⟩. ⟨hal-00829234⟩
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