In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses

Willy Supatto; Delphine Débarre; Bruno Moulia; Eric Brouzés; Jean-Louis Martin; Emmanuel Farge; Emmanuel Beaurepaire

In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses

Willy Supatto ¹ Delphine Débarre ² Bruno Moulia ³ Eric Brouzés ¹ Jean-Louis Martin ² Emmanuel Farge ¹ Emmanuel Beaurepaire ²

1 PCC - Physico-Chimie-Curie

2 LOB - Laboratoire d'optique et biosciences

3 PIAF - Laboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant - Clermont Auvergne

Abstract : The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.

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Journal articles

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https://hal-polytechnique.archives-ouvertes.fr/hal-00829234
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Submitted on : Tuesday, June 18, 2013 - 10:00:34 PM
Last modification on : Thursday, March 21, 2019 - 1:08:32 PM

In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses

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In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses

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