In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses

Abstract : The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.
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Submitted on : Tuesday, June 18, 2013 - 10:00:34 PM
Last modification on : Thursday, March 21, 2019 - 1:08:32 PM

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Willy Supatto, Delphine Débarre, Bruno Moulia, Eric Brouzés, Jean-Louis Martin, et al.. In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2005, 102 (4), pp.1047. ⟨10.1073/pnas.0405316102⟩. ⟨hal-00829234⟩

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