Intraprotein electron transfer and proton dynamics during photoactivation of DNA photolyase from E. coli: Review and new insights from an "inverse" deuterium isotope effect.

Martin Byrdin (1) , Valérie Sartor (1, 2) , André P.M. Eker (3) , Marten H. Vos (4) , Corinne Aubert (5, 1) , Klaus Brettel (1) , Paul Mathis (1)

1 SB2SM - Service de Bioénergétique, Biologie Stucturale, et Mécanismes
2 Université Louis Pasteur - Strasbourg I
3 Department of Cell Biology and Genetics
4 LOB - Laboratoire d'optique et biosciences
5 BIP - Bioénergétique et Ingénierie des Protéines

We review our work on electron transfer and proton dynamics during photoactivation in DNA photolyase from E. coli and discuss a recent theoretical study on this issue. In addition, we present unpublished data on the charge recombination between the fully reduced FADH- and the neutral (deprotonated) radical of the solvent exposed tryptophan W306. We found a pronounced acceleration with decreasing pH and an inverse deuterium isotope effect (kH/kD=0.35 at pL 6.5) and interpret it in a model of a fast protonation equilibrium for the W306 radical. Due to this fast equilibrium, two parallel recombination channels contribute differently at different pH values: one where reprotonation of the W306 radical is followed by electron transfer from FADH- (electron transfer time constant tet in the order of 10-50 µs), and one where electron transfer from FADH- (tet=25 ms) is followed by reprotonation of the W306 anion. Cop. 2004 Elsevier B.V. All rights reserved.

Domains: Life Sciences [q-bio]

Martin Byrdin, Valérie Sartor, André P.M. Eker, Marten H. Vos, Corinne Aubert, et al.. Intraprotein electron transfer and proton dynamics during photoactivation of DNA photolyase from E. coli: Review and new insights from an "inverse" deuterium isotope effect.. Biochimica biophysica acta (BBA) - Bioenergetics, 2004, 1655 (12 april), pp.64. ⟨10.1016/j.bbabio.2003.07.001⟩. ⟨hal-00831846⟩