Skip to Main content Skip to Navigation
Journal articles

An alternative flavin-dependent mechanism for thymidylate synthesis

Abstract : Although deoxythymidylate cannot be provided directly by ribonucleotide reductase, the gene encoding thymidylate synthase ThyA is absent from the genomes of a large number of nonsymbiotic microbes. We show that ThyX (Thy1) proteins of previously unknown function form a large and distinct class of thymidylate synthases. ThyX has a wide but sporadic phylogenetic distribution, almost exclusively limited to microbial genomes lacking thyA. ThyX and ThyA use different reductive mechanisms, because ThyX activity is dependent on reduced flavin nucleotides. Our findings reveal complexity in the evolution of thymidine in present-day DNA. Because ThyX proteins are found in many pathogenic microbes, they present a previously uncharacterized target for antimicrobial compounds.
Complete list of metadatas

https://hal-polytechnique.archives-ouvertes.fr/hal-00836930
Contributor : Denis Roura <>
Submitted on : Tuesday, June 25, 2013 - 3:37:55 PM
Last modification on : Thursday, March 5, 2020 - 6:24:18 PM

Links full text

Identifiers

Collections

Citation

Hannu Myllykallio, Gérard Lipowski, D Leduc, Jonathan Filee, Patrick Forterre, et al.. An alternative flavin-dependent mechanism for thymidylate synthesis. Science, American Association for the Advancement of Science, 2002, 297 (5578), pp.105. ⟨10.1126/science.1072113⟩. ⟨hal-00836930⟩

Share

Metrics

Record views

168