Polarization-resolved second harmonic generation microscopy in biological tissues

Gaël Latour; Ivan Gusachenko; Marie-Claire Schanne-Klein

Polarization-resolved second harmonic generation microscopy in biological tissues

Gaël Latour ¹ Ivan Gusachenko ¹ Marie-Claire Schanne-Klein ¹

1 LOB - Laboratoire d'optique et biosciences

Abstract : In this study, we implemented a polarimetric SHG microscope and analyzed the linear propagation effects that affect polarization-resolved SHG imaging of thick anisotropic collagenous tissues. We developed a theoretical model to account for birefringence and diattenuation along the excitation propagation, and polarization scrambling upon scattering of the harmonic signal. We obtained an excellent agreement with polarization-resolved SHG images at increasing depth within a rat-tail tendon for both polarizations of the forward SHG signal (see Figure 1). Most notably, we observed interference fringes due to birefringence in the SHG depth profile when excited at π/4 angle from the tendon axis.

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Conference papers

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https://hal-polytechnique.archives-ouvertes.fr/hal-00838892
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Submitted on : Wednesday, July 3, 2013 - 3:52:09 PM
Last modification on : Wednesday, January 23, 2019 - 10:29:00 AM

Polarization-resolved second harmonic generation microscopy in biological tissues

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Polarization-resolved second harmonic generation microscopy in biological tissues

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