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Conference papers
Polarization-resolved second harmonic generation microscopy in biological tissues
Abstract : In this study, we implemented a polarimetric SHG microscope and analyzed the linear propagation effects that affect polarization-resolved SHG imaging of thick anisotropic collagenous tissues. We developed a theoretical model to account for birefringence and diattenuation along the excitation propagation, and polarization scrambling upon scattering of the harmonic signal. We obtained an excellent agreement with polarization-resolved SHG images at increasing depth within a rat-tail tendon for both polarizations of the forward SHG signal (see Figure 1). Most notably, we observed interference fringes due to birefringence in the SHG depth profile when excited at π/4 angle from the tendon axis.
https://hal-polytechnique.archives-ouvertes.fr/hal-00838892
Contributor : Denis Roura <>
Submitted on : Wednesday, July 3, 2013 - 3:52:09 PM
Last modification on : Friday, June 18, 2021 - 3:58:03 PM
Contributor : Denis Roura <>
Submitted on : Wednesday, July 3, 2013 - 3:52:09 PM
Last modification on : Friday, June 18, 2021 - 3:58:03 PM