Polarization-resolved second harmonic generation microscopy in biological tissues

Gaël Latour 1 Ivan Gusachenko 1 Marie-Claire Schanne-Klein 1
1 LOB - Laboratoire d'optique et biosciences
Abstract : In this study, we implemented a polarimetric SHG microscope and analyzed the linear propagation effects that affect polarization-resolved SHG imaging of thick anisotropic collagenous tissues. We developed a theoretical model to account for birefringence and diattenuation along the excitation propagation, and polarization scrambling upon scattering of the harmonic signal. We obtained an excellent agreement with polarization-resolved SHG images at increasing depth within a rat-tail tendon for both polarizations of the forward SHG signal (see Figure 1). Most notably, we observed interference fringes due to birefringence in the SHG depth profile when excited at π/4 angle from the tendon axis.
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Submitted on : Wednesday, July 3, 2013 - 3:52:09 PM
Last modification on : Friday, December 6, 2019 - 7:24:09 PM

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CNRS | X-LOB | PARISTECH | X | X-DEP-BIO

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Gaël Latour, Ivan Gusachenko, Marie-Claire Schanne-Klein. Polarization-resolved second harmonic generation microscopy in biological tissues. 2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011, May 2011, Munich, Germany. ⟨10.1109/CLEOE.2011.5942909⟩. ⟨hal-00838892⟩

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