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Photoinduced electron transfer from a novel nanotrigger addressed to the NADPH site within the endothelial NO-synthase to the flavin moieties of the protein
Abstract : We developed a new selective molecular tool to trigger enzymatic activity in a synchronous manner and monitor the sequence of the kinetic events by ultra-fast transient spectroscopy. Our approach is based on a synthetic nanotrigger addressing a selected site within proteins, namely the conserved NADPH binding site common to many enzymes involved in bioreductive processes [1]. The nanotrigger combines a "docking" subunit responsible for the recognition of NADPH sites within proteins and a "chromophoric" subunit responsive to light excitation and able to transfer electrons to the flavin moieties of proteins. We present the first spectroscopic data on such a nanotrigger [1] in the presence of the reductase domain of the endothelial nitric oxide synthase eNOSred.
https://hal-polytechnique.archives-ouvertes.fr/hal-00844508
Contributor : Denis Roura <>
Submitted on : Monday, July 15, 2013 - 2:00:24 PM
Last modification on : Thursday, May 28, 2020 - 11:58:03 AM
Contributor : Denis Roura <>
Submitted on : Monday, July 15, 2013 - 2:00:24 PM
Last modification on : Thursday, May 28, 2020 - 11:58:03 AM