Tunable charge tags for electron-based methods of peptide sequencing: design and applications.

Magdalena Zimnicka 1 Christopher L. Moss 1 Thomas W. Chung 1 Renjie Hui 1, 2 Frantisek Turecek 1
1 Department of Chemistry
2 DCMR - Laboratoire des mécanismes réactionnels
Abstract : Charge tags using basic auxiliary functional groups 6-aminoquinolinylcarboxamido, 4-aminopyrimidyl-1-methylcarboxamido, 2-aminobenzoimidazolyl-1-methylcarboxamido, and the fixed-charge 4-(dimethylamino)pyridyl-1-carboxamido moiety are evaluated as to their properties in electron transfer dissociation mass spectra of arginine C-terminated peptides. The neutral tags have proton affinities that are competitive with those of amino acid residues in peptides. Charge reduction by electron transfer from fluoranthene anion-radicals results in peptide backbone dissociations that improve sequence coverage by providing extensive series of N-terminal c-type fragments without impeding the formation of C-terminal z fragments. Comparison of ETD mass spectra of free and tagged peptides allows one to resolve ambiguities in fragment ion assignment through mass shifts of c ions. Simple chemical procedures are reported for N-terminal tagging of Arg-containing tryptic peptides.
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Journal articles
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https://hal-polytechnique.archives-ouvertes.fr/hal-00904390
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Submitted on : Friday, November 29, 2013 - 3:21:32 PM
Last modification on : Wednesday, March 20, 2019 - 12:12:02 PM

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Magdalena Zimnicka, Christopher L. Moss, Thomas W. Chung, Renjie Hui, Frantisek Turecek. Tunable charge tags for electron-based methods of peptide sequencing: design and applications.. Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2012, 23 (4), pp.608-620. ⟨10.1007/s13361-011-0184-y⟩. ⟨hal-00904390⟩

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