Tunable charge tags for electron-based methods of peptide sequencing: design and applications. - Archive ouverte HAL Access content directly
Journal Articles Journal of The American Society for Mass Spectrometry Year : 2012

Tunable charge tags for electron-based methods of peptide sequencing: design and applications.

(1) , (1) , (1) , (1, 2) , (1)
1
2

Abstract

Charge tags using basic auxiliary functional groups 6-aminoquinolinylcarboxamido, 4-aminopyrimidyl-1-methylcarboxamido, 2-aminobenzoimidazolyl-1-methylcarboxamido, and the fixed-charge 4-(dimethylamino)pyridyl-1-carboxamido moiety are evaluated as to their properties in electron transfer dissociation mass spectra of arginine C-terminated peptides. The neutral tags have proton affinities that are competitive with those of amino acid residues in peptides. Charge reduction by electron transfer from fluoranthene anion-radicals results in peptide backbone dissociations that improve sequence coverage by providing extensive series of N-terminal c-type fragments without impeding the formation of C-terminal z fragments. Comparison of ETD mass spectra of free and tagged peptides allows one to resolve ambiguities in fragment ion assignment through mass shifts of c ions. Simple chemical procedures are reported for N-terminal tagging of Arg-containing tryptic peptides.

Dates and versions

hal-00904390 , version 1 (29-11-2013)

Identifiers

Cite

Magdalena Zimnicka, Christopher L. Moss, Thomas W. Chung, Renjie Hui, Frantisek Turecek. Tunable charge tags for electron-based methods of peptide sequencing: design and applications.. Journal of The American Society for Mass Spectrometry, 2012, 23 (4), pp.608-620. ⟨10.1007/s13361-011-0184-y⟩. ⟨hal-00904390⟩
43 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More