Tunable charge tags for electron-based methods of peptide sequencing: design and applications.

Magdalena Zimnicka; Christopher L. Moss; Thomas W. Chung; Renjie Hui; Frantisek Turecek

Tunable charge tags for electron-based methods of peptide sequencing: design and applications.

Magdalena Zimnicka ¹ Christopher L. Moss ¹ Thomas W. Chung ¹ Renjie Hui ^{1, 2} Frantisek Turecek ¹

2 DCMR - Laboratoire des mécanismes réactionnels

Abstract : Charge tags using basic auxiliary functional groups 6-aminoquinolinylcarboxamido, 4-aminopyrimidyl-1-methylcarboxamido, 2-aminobenzoimidazolyl-1-methylcarboxamido, and the fixed-charge 4-(dimethylamino)pyridyl-1-carboxamido moiety are evaluated as to their properties in electron transfer dissociation mass spectra of arginine C-terminated peptides. The neutral tags have proton affinities that are competitive with those of amino acid residues in peptides. Charge reduction by electron transfer from fluoranthene anion-radicals results in peptide backbone dissociations that improve sequence coverage by providing extensive series of N-terminal c-type fragments without impeding the formation of C-terminal z fragments. Comparison of ETD mass spectra of free and tagged peptides allows one to resolve ambiguities in fragment ion assignment through mass shifts of c ions. Simple chemical procedures are reported for N-terminal tagging of Arg-containing tryptic peptides.

Document type :

Journal articles

Domain :

Chemical Sciences / Theoretical and/or physical chemistry

Complete list of metadatas

https://hal-polytechnique.archives-ouvertes.fr/hal-00904390
Contributor : Denis Roura <>
Submitted on : Friday, November 29, 2013 - 3:21:32 PM
Last modification on : Wednesday, March 20, 2019 - 12:12:02 PM

Tunable charge tags for electron-based methods of peptide sequencing: design and applications.

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Tunable charge tags for electron-based methods of peptide sequencing: design and applications.

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