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Terpenic subfraction of Pterodon pubescens induces apoptosis of K562 leukemic cells by modulating gene expression
Abstract : Deregulation of cell proliferation and apoptosis is linked to malignant cell development. Leukemia is the most frequent cancer in children, and plants are important sources for new potential anti-cancer agents. Although anti-tumoral effects have been shown for Pterodon pubescens extracts, the mechanisms are still obscure. This study describes in Pterodon pubescens a furane diterpene only reported in Pterodon polygalaeflorus, the methyl-6 alpha-acetoxy-7 beta-hydroxyvouacapan-17 beta-oate, indicated by HRMS and (13)C-NMR analysis, and demonstrates some mechanisms of the anti-leukemia action of its terpene subfraction SF5. SF5 induced cytotoxic and anti-proliferative effects on K562 cells. Increased sub-G1 nuclei and Annexin V(+)-FITC cells confirmed apoptosis of leukemic cells by treatment of these cells with SF5. Down-regulation of DNMT1 gene transcription and over-expression of Apaf-1 mRNA suggested that SF5 may be inducing apoptosis of K562 cells by epigenetic up-regulation of pro-apoptotic proteins involved in the mitochondrial intrinsic pathway.
Cited literature [29 references]
https://hal-polytechnique.archives-ouvertes.fr/hal-00951234
Contributor : Denis Roura Connect in order to contact the contributor
Submitted on : Monday, March 3, 2014 - 4:28:55 PM
Last modification on : Thursday, December 9, 2021 - 2:26:02 PM
Long-term archiving on: : Saturday, May 31, 2014 - 10:37:25 AM
Contributor : Denis Roura Connect in order to contact the contributor
Submitted on : Monday, March 3, 2014 - 4:28:55 PM
Last modification on : Thursday, December 9, 2021 - 2:26:02 PM
Long-term archiving on: : Saturday, May 31, 2014 - 10:37:25 AM
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