Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Terpenic subfraction of Pterodon pubescens induces apoptosis of K562 leukemic cells by modulating gene expression

Abstract : Deregulation of cell proliferation and apoptosis is linked to malignant cell development. Leukemia is the most frequent cancer in children, and plants are important sources for new potential anti-cancer agents. Although anti-tumoral effects have been shown for Pterodon pubescens extracts, the mechanisms are still obscure. This study describes in Pterodon pubescens a furane diterpene only reported in Pterodon polygalaeflorus, the methyl-6 alpha-acetoxy-7 beta-hydroxyvouacapan-17 beta-oate, indicated by HRMS and (13)C-NMR analysis, and demonstrates some mechanisms of the anti-leukemia action of its terpene subfraction SF5. SF5 induced cytotoxic and anti-proliferative effects on K562 cells. Increased sub-G1 nuclei and Annexin V(+)-FITC cells confirmed apoptosis of leukemic cells by treatment of these cells with SF5. Down-regulation of DNMT1 gene transcription and over-expression of Apaf-1 mRNA suggested that SF5 may be inducing apoptosis of K562 cells by epigenetic up-regulation of pro-apoptotic proteins involved in the mitochondrial intrinsic pathway.
Document type :
Journal articles
Complete list of metadata

Cited literature [29 references]  Display  Hide  Download

https://hal-polytechnique.archives-ouvertes.fr/hal-00951234
Contributor : Denis Roura Connect in order to contact the contributor
Submitted on : Monday, March 3, 2014 - 4:28:55 PM
Last modification on : Thursday, December 9, 2021 - 2:26:02 PM
Long-term archiving on: : Saturday, May 31, 2014 - 10:37:25 AM

File

or_25_1_215_PDF.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Monica Farah Pereira, Thiago Martino, Sergio Ranto Dalmau, Rodolpho Mattos Albano, Jean-Pierre Férézou, et al.. Terpenic subfraction of Pterodon pubescens induces apoptosis of K562 leukemic cells by modulating gene expression. Oncology Reports, Spandidos Publications, 2011, 25 (1), pp.215-221. ⟨10.3892/or_00001063⟩. ⟨hal-00951234⟩

Share

Metrics

Record views

447

Files downloads

338