Parallel measurements of reaction kinetics using ultralow-volumes

Abstract : We present a new platform for the production and manipulation of microfluidic droplets in view of measuring the evolution of a chemical reaction. Contrary to existing approaches, our device uses gradients of confinement to produce a single drop on demand and guide it to a pre-determined location. In this way, two nanoliter drops containing different reagents can be placed in contact and merged together, in order to trigger a chemical reaction. The reaction rate is extracted from an analysis of the observed reaction-diffusion front. We show that the results obtained using this platform are in excellent agreement with stopped-flow measurements, while decreasing the sample consumption 5000 fold. We also show how the device operation can be parallelized in order to react an initial sample with a range of compounds or concentrations, on a single integrated chip. This integrated chip thus further reduces sample consumption while reducing the time required for the experimental runs from hours to minutes.
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https://hal-polytechnique.archives-ouvertes.fr/hal-00996478
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Submitted on : Thursday, May 29, 2014 - 8:34:01 PM
Last modification on : Wednesday, March 27, 2019 - 4:39:25 PM

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Etienne Fradet, Paul Abbyad, Marten H. Vos, Charles N. Baroud. Parallel measurements of reaction kinetics using ultralow-volumes. Lab on a Chip, Royal Society of Chemistry, 2013, 13 (22), pp.4326-4330. ⟨10.1039/c3lc50768h⟩. ⟨hal-00996478⟩

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