Serum proteins prevent aggregation of Fe2O3 and ZnO nanoparticles

Abstract : Aggregation of metal oxide nanoparticles in aqueous media complicates interpretation of in vitro studies of nanoparticle-cell interactions. We used dynamic light scattering to investigate the aggregation dynamics of iron oxide and zinc oxide nanoparticles. Our results show that iron oxide particles aggregate more readily than zinc oxide particles. Pretreatment with serum stabilises iron oxide and zinc oxide nanoparticles against aggregation. Serum-treated iron oxide is stable only in pure water, while zinc oxide is stable in water or cell culture media. These findings, combined with zeta potential measurements and quantification of proteins adsorbed on particle surface, suggest that serum stabilisation of iron oxide particles occurs primarily through protein adsorption and resulting net surface charge. Zinc oxide stabilisation, however, also involves steric hindrance of particle aggregation. Fluid shear at levels used in flow experiments breaks up iron oxide particle aggregates. These results enhance our understanding of nanoparticle aggregation and its consequences for research on the biological effects of nanomaterials.
Liste complète des métadonnées

https://hal-polytechnique.archives-ouvertes.fr/hal-00996518
Contributeur : Denis Roura <>
Soumis le : mardi 27 mai 2014 - 17:31:15
Dernière modification le : jeudi 11 janvier 2018 - 06:18:25

Identifiants

Collections

Citation

Mark A. Wells, Aamir Abid, Ian M. Kennedy, Abdul I. Barakat. Serum proteins prevent aggregation of Fe2O3 and ZnO nanoparticles. Nanotoxicology, Taylor & Francis, 2012, 6 (8), pp.837-846. 〈10.3109/17435390.2011.625131〉. 〈hal-00996518〉

Partager

Métriques

Consultations de la notice

61