Multicolor two-photon light-sheet microscopy

Pierre Mahou (1) , Julien Vermot (2) , Emmanuel Beaurepaire (1) , Willy Supatto (1)

1 LOB - Laboratoire d'optique et biosciences
2 IGBMC - Institut de génétique et biologie moléculaire et cellulaire

Two-photon microscopy is the most effective approach for deep-tissue fluorescence cellular imaging; however, its application to high-throughput or high-content imaging is often hampered by low pixel rates, challenging multicolor excitation and potential cumulative photodamage. To overcome these limitations, we extended our prior work and combined two-photon scanned light-sheet...

Domaines: Optique [physics.optics]

Pierre Mahou, Julien Vermot, Emmanuel Beaurepaire, Willy Supatto. Multicolor two-photon light-sheet microscopy. Nature Methods, 2014, 11, pp.600-601. ⟨10.1038/nmeth.2963⟩. ⟨hal-01048680⟩