In situ determination of collagen fibrils size via absolute measurements of second harmonic generation signals

Stéphane Bancelin 1 Carole Aimé 2 Ivan Gusachenko 1 Laura Kowalczuk 3 Gael Latour 1 Thibaud Coradin 2 Marie-Claire Schanne-Klein 1
1 LOB - Laboratoire d'Optique et Biosciences
2 MATBIO - Matériaux et Biologie
LCMCP - Laboratoire de Chimie de la Matière Condensée de Paris
3 CRC (UMR_S 872) - Centre de Recherche des Cordeliers
Abstract : We correlated SHG and Electron Microscopies to calibrate SHG signals as a function of collagen fibril diameter, down to 30 nm. We observed a fourth power dependence, in agreement with analytical and numerical calculations. We applied this calibration to abnormal fibrils in the Descemet’s membrane of intact unfixed rat corneas.
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https://hal-polytechnique.archives-ouvertes.fr/hal-01175452
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Submitted on : Friday, July 10, 2015 - 2:45:33 PM
Last modification on : Tuesday, May 28, 2019 - 1:44:38 AM

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Stéphane Bancelin, Carole Aimé, Ivan Gusachenko, Laura Kowalczuk, Gael Latour, et al.. In situ determination of collagen fibrils size via absolute measurements of second harmonic generation signals. European Conferences on Biomedical Optics (ECBO) 2015, Jun 2015, Munich, Germany. ⟨hal-01175452⟩

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