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Conference papers
In situ determination of collagen fibrils size via absolute measurements of second harmonic generation signals
Stéphane Bancelin
1
Carole Aimé
2
Ivan Gusachenko
1
Laura Kowalczuk
3
Gael Latour
1
Thibaud Coradin
2
Marie-Claire Schanne-Klein
1
Gael Latour 1
Author
Thibaud Coradin 2
Author IdHAL : thibaud-coradin ORCID : https://orcid.org/0000-0003-3374-5722
Marie-Claire Schanne-Klein 1
Author IdHAL : marie-claire-schanne-klein ORCID : https://orcid.org/0000-0003-3026-8932
1
LOB - Laboratoire d'Optique et Biosciences (Ecole Polytechnique
91128 PALAISEAU Cedex - France)
- X - École polytechnique (École polytechnique, 91128 Palaiseau Cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1182 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7645 (France)
2
MATBIO - Matériaux et Biologie (France)
- LCMCP - Laboratoire de Chimie de la Matière Condensée de Paris (11 place Marcelin Berthelot 75231 Paris Cedex 05 - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- CdF (institution) - Collège de France (France)
- CNRS - Centre National de la Recherche Scientifique : UMR7574 (France)
3
CRC (UMR_S 872) - Centre de Recherche des Cordeliers (CRBM des Cordeliers 15, rue de l'ecole de medecine batiment E 75270 Paris cedex 06 - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- UPD5 - Université Paris Descartes - Paris 5 : U872 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U872 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 872 (France)
Abstract : We correlated SHG and Electron Microscopies to calibrate SHG signals as a function of collagen fibril diameter, down to 30 nm. We observed a fourth power dependence, in agreement with analytical and numerical calculations. We applied this calibration to abnormal fibrils in the Descemet’s membrane of intact unfixed rat corneas.
Document type :
Conference papers
Complete list of metadatas
https://hal-polytechnique.archives-ouvertes.fr/hal-01175452
Contributor : Denis Roura <>
Submitted on : Friday, July 10, 2015 - 2:45:33 PM
Last modification on : Tuesday, October 6, 2020 - 11:38:02 AM
Contributor : Denis Roura <>
Submitted on : Friday, July 10, 2015 - 2:45:33 PM
Last modification on : Tuesday, October 6, 2020 - 11:38:02 AM
Identifiers
- HAL Id : hal-01175452, version 1
Collections
X | CNRS | LCMCP | X-LOB | UPMC | CORDELIERS | CDF | UNIV-PARIS5 | INC-CNRS | LCMCP-MATBIO | USPC | UNIV-PARIS-SACLAY | X-DEP-BIO | SORBONNE-UNIVERSITE | SU-SCIENCES | UNIV-PARIS | PSL
Citation
Stéphane Bancelin, Carole Aimé, Ivan Gusachenko, Laura Kowalczuk, Gael Latour, et al.. In situ determination of collagen fibrils size via absolute measurements of second harmonic generation signals. European Conferences on Biomedical Optics (ECBO) 2015, Jun 2015, Munich, Germany. ⟨hal-01175452⟩
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