In situ determination of collagen fibrils size via absolute measurements of second harmonic generation signals

Stéphane Bancelin (1) , Carole Aimé (2) , Ivan Gusachenko (1) , Laura Kowalczuk (3) , Gael Latour (1) , Thibaud Coradin (2) , Marie-Claire Schanne-Klein (1)

1 LOB - Laboratoire d'Optique et Biosciences
2 MATBIO - Matériaux et Biologie
3 CRC (UMR_S 872) - Centre de Recherche des Cordeliers

We correlated SHG and Electron Microscopies to calibrate SHG signals as a function of collagen fibril diameter, down to 30 nm. We observed a fourth power dependence, in agreement with analytical and numerical calculations. We applied this calibration to abnormal fibrils in the Descemet’s membrane of intact unfixed rat corneas.

Domains: Biophysics; Optics [physics.optics]; Imaging

Stéphane Bancelin, Carole Aimé, Ivan Gusachenko, Laura Kowalczuk, Gael Latour, et al.. In situ determination of collagen fibrils size via absolute measurements of second harmonic generation signals. European Conferences on Biomedical Optics (ECBO) 2015, Jun 2015, Munich, Germany. ⟨hal-01175452⟩