Membrane protrusion powers clathrin-independent endocytosis of interleukin-2 receptor.

Abstract : Endocytosis controls many functions including nutrient uptake, cell division, migration and signal transduction. A clathrin- and caveolin-independent endocytosis pathway is used by important physiological cargos, including interleukin-2 receptors (IL-2R). However, this process lacks morphological and dynamic data. Our electron microscopy (EM) and tomography studies reveal that IL-2R-pits and vesicles are initiated at the base of protrusions. We identify the WAVE complex as a specific endocytic actor. The WAVE complex interacts with IL-2R, via a WAVE-interacting receptor sequence (WIRS) present in the receptor polypeptide, and allows for receptor clustering close to membrane protrusions. In addition, using total internal reflection fluorescent microscopy (TIRF) and automated analysis we demonstrate that two timely distinct bursts of actin polymerization are required during IL-2R uptake, promoted first by the WAVE complex and then by N-WASP. Finally, our data reveal that dynamin acts as a transition controller for the recruitment of Arp2/3 activators required for IL-2R endocytosis. Altogether, our work identifies the spatio-temporal specific role of factors initiating clathrin-independent endocytosis by a unique mechanism that does not depend on the deformation of a flat membrane, but rather on that of membrane protrusions.
Type de document :
Article dans une revue
EMBO Journal, EMBO Press, 2015, 34 (16), pp.2147-61. 〈10.15252/embj.201490788〉
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Soumis le : lundi 21 septembre 2015 - 21:27:17
Dernière modification le : dimanche 2 décembre 2018 - 01:24:11

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Cyril Basquin, Michaël Trichet, Helena Vihinen, Valérie Malardé, Thibault Lagache, et al.. Membrane protrusion powers clathrin-independent endocytosis of interleukin-2 receptor.. EMBO Journal, EMBO Press, 2015, 34 (16), pp.2147-61. 〈10.15252/embj.201490788〉. 〈hal-01202886〉



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