Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection - École polytechnique Accéder directement au contenu
Article Dans Une Revue Applied Physics Letters Année : 2015

Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection

Résumé

Using Attenuated Total Reflection imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of epithelial cells by saponin. The terahertz signal is mostly sensitive to the intracellular protein concentration in the cells, in a very good agreement with standard bicinchoninic acid protein measurements. It opens the way to in situ real time dynamics of protein content and permeabilization in live cells.
Fichier principal
Vignette du fichier
14930168.pdf (957.63 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01222843 , version 1 (30-10-2015)

Identifiants

Citer

Marianne Grognot, Guilhem Gallot. Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection. Applied Physics Letters, 2015, 107 (10), pp.103702. ⟨10.1063/1.4930168⟩. ⟨hal-01222843⟩
295 Consultations
767 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More