ROS Detection and Quantification with Lanthanide-Based Nanosensors

Mouna Abdesselem (1) , Thierry Gacoin (2) , Jean-Pierre Boilot (2) , Pierre-Louis Tharaux (3) , Antigoni Alexandrou (1) , Cedric I. Bouzigues (1)

1 LOB - Laboratoire d'optique et biosciences
2 LPMC - Laboratoire de physique de la matière condensée
3 PARCC - UMR-S U970 - Paris-Centre de Recherche Cardiovasculaire

Reactive oxygen species (ROS), in particular hydrogen peroxide (H2O2), are produced as secondary cellular messengers involved in a variety of signaling pathways. Detecting ROS concentration in cells and in living organisms is particularly important because ROS oxidative stress plays a complex role in multiple pathologies: inflammatory processes, neurodegenerative diseases, and cancer. We first report on an europium-based H2O2 sensor (Y0.6Eu0.4VO4) that shows red, oxidant-dependant, reversible luminescence modulation used in cells in culture.

Domaines: Optique [physics.optics]

Mouna Abdesselem, Thierry Gacoin, Jean-Pierre Boilot, Pierre-Louis Tharaux, Antigoni Alexandrou, et al.. ROS Detection and Quantification with Lanthanide-Based Nanosensors. 59th Annual Meeting of the Biophysical-Society, Feb 2015, Baltimore, United States. Biophysical Journal, 108 (2), pp.483a, 2015, ⟨10.1016/j.bpj.2014.11.2640⟩. ⟨hal-01237532⟩