Ultrafast conformational changes in biomolecules studied by time-resolved circular dichroism

Abstract : Circular dichroism (CD) is known to be a very sensitive probe of molecular conformation, and it is in particular widely used in biochemistry. Measuring the CD as a function of time is therefore very appealing to access information on the dynamics of conformational changes in molecules or biomolecules. We have implemented such a time-resolved experiments in two complementary configurations: a sub-picosecond pump-probe one and a microsecond detection of CD coupled to a T-jump experiment. We present two experiments based on these techniques: the ultrafast motion of the carbonyl group in the chromophore of the Photoactive Yellow Protein after photoexcitation and the dynamics of thermal denaturation in model peptides. © (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII, Oct 2015, Toulouse, France. SPIE Proceedings, 9652, Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII. 〈10.1117/12.2216989〉
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Soumis le : jeudi 7 avril 2016 - 21:48:18
Dernière modification le : jeudi 10 mai 2018 - 02:08:14

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Pascale Changenet-Barret, François Hache. Ultrafast conformational changes in biomolecules studied by time-resolved circular dichroism . Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII, Oct 2015, Toulouse, France. SPIE Proceedings, 9652, Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII. 〈10.1117/12.2216989〉. 〈hal-01299611〉

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