Multiphoton microscopy of engineered dermal substitutes: assessment of 3-D collagen matrix remodeling induced by fibroblast contraction.

Abstract : Dermal fibroblasts are responsible for the generation of mechanical forces within their surrounding extracellular matrix and can be potentially targeted by anti-aging ingredients. Investigation of the modulation of fibroblast contraction by these ingredients requires the implementation of three-dimensional in situ imaging methodologies. We use multiphoton microscopy to visualize unstained engineered dermal tissue by combining second-harmonic generation that reveals specifically fibrillar collagen and two-photon excited fluorescence from endogenous cellular chromophores. We study the fibroblast-induced reorganization of the collagen matrix and quantitatively evaluate the effect of Y-27632, a RhoA-kinase inhibitor, on dermal substitute contraction. We observe that collagen fibrils rearrange around fibroblasts with increasing density in control samples, whereas collagen fibrils show no remodeling in the samples containing the RhoA-kinase inhibitor. Moreover, we show that the inhibitory effects are reversible. Our study demonstrates the relevance of multiphoton microscopy to visualize three-dimensional remodeling of the extracellular matrix induced by fibroblast contraction or other processes.
Type de document :
Article dans une revue
Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2010, 15 (5), 〈10.1117/1.3503411〉
Liste complète des métadonnées

https://hal-polytechnique.archives-ouvertes.fr/hal-01619016
Contributeur : Denis Roura <>
Soumis le : mercredi 18 octobre 2017 - 21:21:56
Dernière modification le : jeudi 10 mai 2018 - 02:08:03

Lien texte intégral

Identifiants

Collections

Citation

Ana-Maria Pena, Dominique Fagot, Christian Olive, Jean-François Michelet, Jean-Baptiste Galey, et al.. Multiphoton microscopy of engineered dermal substitutes: assessment of 3-D collagen matrix remodeling induced by fibroblast contraction.. Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2010, 15 (5), 〈10.1117/1.3503411〉. 〈hal-01619016〉

Partager

Métriques

Consultations de la notice

118