The influence of manufacturing and alterations on skin-based artifacts as characterized by nonlinear optical microscopy

Laurianne Robinet 1, * Sylvie Thao 2 Schanne-Klein Marie-Claire 3 Gaël Latour 4
* Corresponding author
1 CRC - Centre de Recherche sur la Conservation
2 CRCC - Centre de Recherche pour la Conservation des Collections
CRC - Centre de Recherche sur la Conservation
3 LOB - Laboratoire d'Optique et Biosciences
4 IMNC (UMR_8165) - Imagerie et Modélisation en Neurobiologie et Cancérologie
Abstract : Nonlinear optical (NLO) microscopy is an efficient technique to investigate artworks as it allows non-invasive, three-dimensional imaging with micrometer-scale resolution. This technique can combine two-photon excitation fluorescence (2PEF) from exogenous and endogenous fluorophores and second harmonic generation (SHG), which enables the visualization of unstained fibrillar collagen. The application of NLO microscopy to different skin-based materials encountered in cultural heritage showed the influence of manufacturing, in particular the type of tannin and fats introduced, on the morphological structure and collected signals. The data also showed that NLO microscopy was well-suited to non-invasive examination of the degradation state of collagen in all skinbased materials, particularly based on the SHG images, and could be used to identify artifacts most at risk in collections so suitable conservation measures could be taken.
Keywords : collagen alum-tawed skin leather parchment nonlinear optical microscopy noninvasive imaging alteration
Document type :
Conference papers
Complete list of metadatas
https://hal-polytechnique.archives-ouvertes.fr/hal-01761403
Contributor : Aurélien Arnoux <>
Submitted on : Monday, April 9, 2018 - 10:17:09 AM
Last modification on : Thursday, January 16, 2020 - 10:10:07 AM

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  • HAL Id : hal-01761403, version 1

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X | CNRS | IMNC | X-LOB | PARISTECH | IN2P3 | UNIV-PARIS-SACLAY | X-DEP-BIO | CONSERVATION | UNIV-PARIS7 | USPC | MNHN

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Laurianne Robinet, Sylvie Thao, Schanne-Klein Marie-Claire, Gaël Latour. The influence of manufacturing and alterations on skin-based artifacts as characterized by nonlinear optical microscopy . ICOM-CC 18th Triennial Conference 2017 Copenhagen, Sep 2017, Copenhague, Denmark. pp.1609. ⟨hal-01761403⟩

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