A unique surface on Pat1 C-terminal domain directly interacts with Dcp2 decapping enzyme and Xrn1 5′–3′ mRNA exonuclease in yeast

Clément Charenton 1 Claudine Gaudon-Plesse 2, 3 Zaineb Fourati 1 Valério Taverniti 2, 3 Régis Back 1 Olga Kolesnikova 2, 3 Bertrand Séraphin 2, 3, * Marc Graille 1, *
* Corresponding author
1 BIOC - Laboratoire de Biochimie de l'Ecole polytechnique
2 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
3 UNISTRA - Université de Strasbourg
Abstract : The Pat1 protein is a central player of eukaryotic mRNA decay that has also been implicated in translational control. It is commonly considered a central platform responsible for the recruitment of several RNA decay factors. We demonstrate here that a yeast-specific C-terminal region from Pat1 interacts with several short motifs, named helical leucine-rich motifs (HLMs), spread in the long C-terminal region of yeast Dcp2 decapping enzyme. Structures of Pat1-HLM complexes reveal the basis for HLM recognition by Pat1. We also identify a HLM present in yeast Xrn1, the main 5'-3' exonuclease involved in mRNA decay. We show further that the ability of yeast Pat1 to bind HLMs is required for efficient growth and normal mRNA decay. Overall, our analyses indicate that yeast Pat1 uses a single binding surface to successively recruit several mRNA decay factors and show that interaction between those factors is highly polymorphic between species.
Keywords : Eukaryotic mRNA decay mRNA decapping Protein–protein interaction Yeast
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Submitted on : Wednesday, December 12, 2018 - 3:35:28 PM
Last modification on : Friday, June 28, 2019 - 9:38:04 AM

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Clément Charenton, Claudine Gaudon-Plesse, Zaineb Fourati, Valério Taverniti, Régis Back, et al.. A unique surface on Pat1 C-terminal domain directly interacts with Dcp2 decapping enzyme and Xrn1 5′–3′ mRNA exonuclease in yeast. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2017, 114 (45), pp.E9493-E9501. ⟨10.1073/pnas.1711680114⟩. ⟨hal-01953029⟩

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