 |
École polytechnique Open archive |  |
 |
Journal articles
Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy
Abstract : Large-scale microscopy approaches are transforming brain imaging, but currently lack efficient multicolor contrast modalities. We introduce chromatic multiphoton serial (ChroMS) microscopy, a method integrating one-shot multicolor multiphoton excitation through wavelength mixing and serial block-face image acquisition. This approach provides organ-scale micrometric imaging of spectrally distinct fluorescent proteins and label-free nonlinear signals with constant micrometer-scale resolution and sub-micron channel registration over the entire imaged volume. We demonstrate tridimensional (3D) multicolor imaging over several cubic millimeters as well as brain-wide serial 2D multichannel imaging. We illustrate the strengths of this method through color-based 3D analysis of astrocyte morphology and contacts in the mouse cerebral cortex, tracing of individual pyramidal neurons within densely Brainbow-labeled tissue, and multiplexed whole-brain mapping of axonal projections labeled with spectrally distinct tracers. ChroMS will be an asset for multiscale and system-level studies in neuroscience and beyond.
Cited literature [68 references]
https://hal-polytechnique.archives-ouvertes.fr/hal-02114066
Contributor : Aurélien Arnoux <>
Submitted on : Wednesday, November 20, 2019 - 2:50:50 PM
Last modification on : Thursday, March 5, 2020 - 7:07:21 PM
Contributor : Aurélien Arnoux <>
Submitted on : Wednesday, November 20, 2019 - 2:50:50 PM
Last modification on : Thursday, March 5, 2020 - 7:07:21 PM
File
s41467-019-09552-9.pdf
Publisher files allowed on an open archive
Licence
Distributed under a Creative Commons Attribution 4.0 International License
