Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics

Stéphane Bancelin (1, 2) , Barbara Lynch (3, 4) , Christelle Bonod-Bidaud (5, 6) , Petr Dokládal (7) , Florence Ruggiero (5) , Jean-Marc Allain (3, 8) , Marie-Claire Schanne-Klein (1)

1 LOB - Laboratoire d'Optique et Biosciences
2 IINS - Institut Interdisciplinaire de Neurosciences
3 LMS - Laboratoire de mécanique des solides
4 L'Oréal Recherche - L'Oréal Recherche France
5 IGFL - Institut de Génomique Fonctionnelle de Lyon
6 LBTI - Laboratoire de Biologie Tissulaire et d'ingénierie Thérapeutique UMR 5305
7 CMM - Centre de Morphologie Mathématique
8 M3DISIM - Mathematical and Mechanical Modeling with Data Interaction in Simulations for Medicine

An important issue in tissue biomechanics is to decipher the relationship between the mechanical behavior at macroscopic scale and the organization of the collagen fiber network at microscopic scale. Here, we present a protocol to combine traction assays with multiphoton microscopy in ex vivo murine skin. This multiscale approach provides simultaneously the stress/stretch response of a skin biopsy and the collagen reorganization in the dermis by use of second harmonic generation (SHG) signals and appropriate image processing.

Keywords: Multiphoton microscopy; Biomechanics; Collagen; Skin

Domains: Optics [physics.optics]; Imaging; Dermatology; Biomechanics [physics.med-ph]

Stéphane Bancelin, Barbara Lynch, Christelle Bonod-Bidaud, Petr Dokládal, Florence Ruggiero, et al.. Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics. Sagi, Irit; Afratis, Nikolaos A. Collagen: Methods and Protocols, 1944, Humana Press, pp.145-155, 2019, Methods in Molecular Biology, 978-1-4939-9095-5. ⟨10.1007/978-1-4939-9095-5_11⟩. ⟨hal-02115604⟩