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NMR solution and X-ray crystal structures of a DNA molecule containing both right- and left-handed parallel-stranded G-quadruplexes

Abstract : Analogous to the B- and Z-DNA structures in double-helix DNA, there exist both right- and left-handed quadruple-helix (G-quadruplex) DNA. Numerous conformations of right-handed and a few left-handed G-quadruplexes were previously observed, yet they were always identified separately. Here, we present the NMR solution and X-ray crystal structures of a right- and left-handed hybrid G-quadruplex. The structure reveals a stacking interaction between two G-quadruplex blocks with different helical orientations and displays features of both right- and left-handed G-quadruplexes. An analysis of loop mutations suggests that single-nucleotide loops are preferred or even required for the left-handed G-quadruplex formation. The discovery of a right- and left-handed hybrid G-quadruplex further expands the polymorphism of G-quadruplexes and is potentially useful in designing a left-to-right junction in G-quadruplex engineering.
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https://hal-polytechnique.archives-ouvertes.fr/hal-02163879
Contributor : Aurélien Arnoux <>
Submitted on : Friday, November 27, 2020 - 1:26:37 PM
Last modification on : Saturday, November 28, 2020 - 3:32:11 AM
Long-term archiving on: : Sunday, February 28, 2021 - 7:30:10 PM

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Fernaldo Richtia Winnerdy, Blaž Bakalar, Arijit Maity, J Jeya Vandana, Yves Mechulam, et al.. NMR solution and X-ray crystal structures of a DNA molecule containing both right- and left-handed parallel-stranded G-quadruplexes. Nucleic Acids Research, Oxford University Press, 2019, 47, pp.gkz349. ⟨10.1093/nar/gkz349⟩. ⟨hal-02163879⟩

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