https://hal-polytechnique.archives-ouvertes.fr/hal-02163879Winnerdy, Fernaldo RichtiaFernaldo RichtiaWinnerdyDivision of Mathematical Sciences, School of Physical and Mathematical Sciences, College of Science - Nanyang Technological University [Singapour]Bakalar, BlažBlažBakalarDivision of Mathematical Sciences, School of Physical and Mathematical Sciences, College of Science - Nanyang Technological University [Singapour]Maity, ArijitArijitMaityDivision of Mathematical Sciences, School of Physical and Mathematical Sciences, College of Science - Nanyang Technological University [Singapour]Vandana, J JeyaJ JeyaVandanaDivision of Mathematical Sciences, School of Physical and Mathematical Sciences, College of Science - Nanyang Technological University [Singapour]Mechulam, YvesYvesMechulamBIOC - Laboratoire de Biochimie de l'Ecole polytechnique - X - École polytechnique - CNRS - Centre National de la Recherche ScientifiqueSchmitt, EmmanuelleEmmanuelleSchmittBIOC - Laboratoire de Biochimie de l'Ecole polytechnique - X - École polytechnique - CNRS - Centre National de la Recherche ScientifiquePhan, Anh TuânAnh TuânPhanDivision of Mathematical Sciences, School of Physical and Mathematical Sciences, College of Science - Nanyang Technological University [Singapour]NMR solution and X-ray crystal structures of a DNA molecule containing both right- and left-handed parallel-stranded G-quadruplexesHAL CCSD2019[SDV.BC] Life Sciences [q-bio]/Cellular Biology[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology[SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM][SDV.CAN] Life Sciences [q-bio]/CancerArnoux, Aurélien2020-11-27 13:26:372020-11-28 03:32:112020-11-27 13:56:00enJournal articleshttps://hal-polytechnique.archives-ouvertes.fr/hal-02163879/document10.1093/nar/gkz349application/pdf1Analogous to the B- and Z-DNA structures in double-helix DNA, there exist both right- and left-handed quadruple-helix (G-quadruplex) DNA. Numerous conformations of right-handed and a few left-handed G-quadruplexes were previously observed, yet they were always identified separately. Here, we present the NMR solution and X-ray crystal structures of a right- and left-handed hybrid G-quadruplex. The structure reveals a stacking interaction between two G-quadruplex blocks with different helical orientations and displays features of both right- and left-handed G-quadruplexes. An analysis of loop mutations suggests that single-nucleotide loops are preferred or even required for the left-handed G-quadruplex formation. The discovery of a right- and left-handed hybrid G-quadruplex further expands the polymorphism of G-quadruplexes and is potentially useful in designing a left-to-right junction in G-quadruplex engineering.