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A Minimal Sequence for Left-Handed G-Quadruplex Formation

Abstract : Recently, we observed the first example of a left-handed G-quadruplex structure formed by natural DNA, named Z-G4. We analysed the Z-G4 structure and inspected its primary 28-nt sequence in order to identify motifs that convey the unique left-handed twist. Using circular dichroism spectroscopy, NMR spectroscopy, and X-ray crystallography, we revealed a minimal sequence motif of 12 nt (GTGGTGGTGGTG) for formation of the left-handed DNA G-quadruplex, which is found to be highly abundant in the human genome. A systematic analysis of thymine loop mutations revealed a moderate sequence tolerance, which would further broaden the space of sequences prone to left-handed G-quadruplex formation.
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Submitted on : Tuesday, June 25, 2019 - 9:18:26 AM
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Blaž Bakalar, Brahim Heddi, Emmanuelle Schmitt, Yves Mechulam, Anh Tuân Phan. A Minimal Sequence for Left-Handed G-Quadruplex Formation. Angewandte Chemie International Edition, Wiley-VCH Verlag, 2019, 58 (8), pp.2331-2335. ⟨10.1002/anie.201812628⟩. ⟨hal-02164372⟩