A novel minimal motif for left-handed G-quadruplex formation

Poulomi Das (1) , Fernaldo Richtia Winnerdy (1) , Arijit Maity (1) , Yves Mechulam (2) , Anh Tuân Phan (1)

1 Nanyang Technological University [Singapour]
2 BIOC - Laboratoire de Biologie Structurale de la Cellule

A recent study on the left-handed G-quadruplex (LHG4) DNA revealed a 12-nt minimal motif GTGGTGGTGGTG with the ability to independently form an LHG4 and to drive an adjacent sequence to LHG4 formation. Here we have identified a second LHG4-forming motif, GGTGGTGGTGTG, and determined the X-ray crystal structure of an LHG4 involving this motif. Our structural analysis indicated the role of split guanines and single thymine loops in promoting LHG4 formation.

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology; Structural Biology [q-bio.BM]

Poulomi Das, Fernaldo Richtia Winnerdy, Arijit Maity, Yves Mechulam, Anh Tuân Phan. A novel minimal motif for left-handed G-quadruplex formation. Chemical Communications, 2021, 57 (20), pp.2527-2530. ⟨10.1039/d0cc08146a⟩. ⟨hal-03188419⟩