Bulges in left-handed G-quadruplexes

Poulomi Das (1) , Khac Huy Ngo (1) , Fernaldo richtia Winnerdy (1) , Arijit Maity (1) , Blaž Bakalar (1) , Yves Mechulam (2) , Emmanuelle Schmitt (2) , Anh Tuân Phan (1)

1 Nanyang Technological University [Singapour]
2 BIOC - Laboratoire de Biologie Structurale de la Cellule

G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures.

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology; Structural Biology [q-bio.BM]

Poulomi Das, Khac Huy Ngo, Fernaldo richtia Winnerdy, Arijit Maity, Blaž Bakalar, et al.. Bulges in left-handed G-quadruplexes. Nucleic Acids Research, 2021, 49 (3), pp.1724-1736. ⟨10.1093/nar/gkaa1259⟩. ⟨hal-03188426⟩