I. La-figure, 18 présente un échantillon ST provenant de l'artère carotide d'un Rat mâle témoin. Les figures IV)C.19 et IV)C.20 présentent les images d'un échantillon de l'artère carotide provenant de Rats descendants d'un père traité. Nous observons que les feuillets d'élastine sont irrégulièrement espacés et présentent des ondulations (figure IV)C

L. Figures and I. , 21 et 22 montrent respectivement des coupes ST d'aortes d'animaux témoin et descendant de père traité. Les feuillets d'élastine sont particulièrement ondulés et sectionnés (figure IV)C.22) chez les descendants par comparaison avec l'aorte des témoins

E. Adler, Nonlinear Optical Frequency Polarization in a Dielectric, Physical Review, vol.134, issue.3A, p.728, 1964.
DOI : 10.1103/PhysRev.134.A728

M. I. Angelova and D. S. Dimitrov, Liposome electroformation, Faraday Discuss, Chem. Soc, vol.81, pp.300-311, 1986.

A. Y. Resniansky and A. P. Shkurinov, Ultrafast Processes in spectroscopy, Svelto et al, 1996.

M. A. Belkin, S. H. Han, X. Wei, and Y. R. Shen, Sum-Frequency Generation in Chiral Liquids near Electronic Resonance, Physical Review Letters, vol.87, issue.11, p.11301, 2001.
DOI : 10.1103/PhysRevLett.87.113001

E. Beaurepaire, L. Moreaux, F. Amblard, and J. Mertz, Combined scanning optical coherence and two-photon-excited fluorescence microscopy, Optics Letters, vol.24, issue.14, pp.969-971, 1999.
DOI : 10.1364/OL.24.000969

P. Bornstein and H. Sage, Structurally Distinct Collagen Types, Annual Review of Biochemistry, vol.49, issue.1, pp.957-1003, 1980.
DOI : 10.1146/annurev.bi.49.070180.004521

O. Bouevitch, A. Lewis, I. Pinevsky, . Wuksell, and L. Loew, Probing membrane potential with nonlinear optics, Biophysical Journal, vol.65, issue.2, pp.672-679, 1993.
DOI : 10.1016/S0006-3495(93)81126-3

M. Vogel, R. H. Fink, and D. Uttenweiler, Second harmonic generation imaging in muscle fibers, Proceedings of SPIE-OSA Biomedical Optics, pp.112-120, 2003.

W. R. Ware, S. K. Lee, G. J. Brant, and P. P. Chow, Nanosecond Time???Resolved Emission Spectroscopy: Spectral Shifts due to Solvent???Excited Solute Relaxation, The Journal of Chemical Physics, vol.54, issue.11, pp.4729-4737, 1971.
DOI : 10.1063/1.1674748

J. D. Byers, H. I. Yee, and J. M. Hicks, A second harmonic generation analog of optical rotatory dispersion for the study of chiral monolayers, The Journal of Chemical Physics, vol.101, issue.7, pp.6233-6241, 1994.
DOI : 10.1063/1.468378

P. J. Campagola, M. Wei, A. Lewis, and L. Loew, High-Resolution Nonlinear Optical Imaging of Live Cells by Second Harmonic Generation, Biophysical Journal, vol.77, issue.6, pp.3341-349, 1999.
DOI : 10.1016/S0006-3495(99)77165-1

M. A. Cattell, J. C. Anderson, and P. S. Hasleton, Age-related changes in amounts and concentrations of collagen and elastin in normotensive human thoracic aorta, Clinica Chimica Acta, vol.245, issue.1, pp.73-84, 1996.
DOI : 10.1016/0009-8981(95)06174-6

M. Cautno and J. Squire, Restingmyosin cross-bridge configuration in frog muscle thck filament, J. Cell. Biol, vol.102, pp.610-618, 1986.

C. Alimentarius, Codex Alimentarius Commission, Consideration of intake of pesticide residues: Reports on pesticide residue intake studies at international and national level based on revised guidelines for predicting dietary intake of pesticide residues, Reports of 13TH session of the Codex committee on pesticide residues, 1998.

M. J. Crawford, S. Haslam, J. M. Probert, Y. A. Gruzdkov, and J. G. Frey, Second harmonic generation from the air/water interface of an aqueous solution of the dipeptide Boc-Trp-Trp, Chemical Physics Letters, vol.230, issue.3, p.260, 1994.
DOI : 10.1016/0009-2614(94)01142-7

Z. Deyl, K. Macek, M. Adam, and O. Vancikova, Studies on the chemical nature of elastin fluorescence, Biochimica et Biophysica Acta (BBA) - Protein Structure, vol.625, issue.2, pp.248-254, 1980.
DOI : 10.1016/0005-2795(80)90288-3

D. A. Dombeck, M. Blanchard-desce, and W. W. Webb, Optical Recording of Action Potentials with Second-Harmonic Generation Microscopy, Journal of Neuroscience, vol.24, issue.4, pp.999-1003, 2004.
DOI : 10.1523/JNEUROSCI.4840-03.2004

D. R. Eyre, Collagen: molecular diversity in the body's protein scaffold, Science, vol.207, issue.4437, p.207, 1980.
DOI : 10.1126/science.7355290

S. Fine and W. P. Hansen, Optical Second Harmonic Generation in Biological Systems, Applied Optics, vol.10, issue.10, pp.2350-2353, 1971.
DOI : 10.1364/AO.10.002350

. Göppert-mayer, Göppert-Mayer, über Elementarakte mit zwei quantensprüngen, Annalen der Physik, vol.9, pp.273-294, 1931.

A. Alfano and R. R. , Second-harmonic tomography of tissues, Opt. Lett, vol.22, issue.17, pp.1323-1325, 1997.

P. Guyot-sionnest-guyot-sionnest, W. Chen, and Y. R. Shen, Local and nonlocal surface nonlinearities for surface optical second-harmonic generation, Physical Review B, vol.35, issue.9, pp.4420-4446, 1986.
DOI : 10.1103/PhysRevB.35.4420

P. Guyot-sionnest-guyot-sionnest and Y. R. Shen, Bulk contribution in surface second-harmonic generation, Physical Review B, vol.38, issue.12, pp.7985-89, 1988.
DOI : 10.1103/PhysRevB.38.7985

F. Hache, H. Mesnil, and M. C. Schanne-klein, Application of classical models of chirality to surface second harmonic generation, The Journal of Chemical Physics, vol.115, issue.14, p.6707, 2001.
DOI : 10.1063/1.1404983

URL : https://hal.archives-ouvertes.fr/hal-00837033

L. Hecht and L. D. Barron, New aspects of second-harmonic optical activity from chiral surfaces and interfaces, Molecular Phys, p.61, 1996.

T. F. Heinz, H. W. Tom, and Y. R. Shen, Determination of molecular orientation of monolayer adsorbates by optical second-harmonic generation, Physical Review A, vol.28, issue.3, p.1883, 1983.
DOI : 10.1103/PhysRevA.28.1883

T. F. Heinz, Non Linear Electromagnetic Phenomena, 1991.

W. Hornebek and L. Robert, Elastase Type Protcases of the Arterial Wall : their Properties and Biological Role, Biologie and Pathology of Platelet-Vessel Wall Interactions, pp.124-144, 1987.

S. H. Huang, A. A. Heikal, and W. W. Webb, Two-Photon Fluorescence Spectroscopy and Microscopy of NAD(P)H and Flavoprotein, Biophysical Journal, vol.82, issue.5, pp.2811-2825, 2002.
DOI : 10.1016/S0006-3495(02)75621-X

M. Kauranen, T. Verbiest, J. J. Maki, and A. Persoons, Quantitative determination of electric and magnetic second-order susceptibility tensors of chiral surfaces, Physical Review B, vol.55, issue.4, p.1985, 1994.
DOI : 10.1103/PhysRevB.55.R1985

M. Kauranen, T. Verbiest, and A. Persoons, Second-order nonlinear optical signatures of surface chirality, Journal of Modern Optics, vol.8, issue.2, pp.403-424, 1998.
DOI : 10.1063/1.460861

W. Kauzman, Quantum Chemistry, Academic press, 1957.

F. Keeley, Cours sur l'élastine, 2004.

C. M. Kielty, M. Lees, C. A. Shuttleworth, and D. Woolley, Catabolism of Intact Type VI Collagen Microfibrils: Susceptibility to Degradation by Serine Proteinases, Biochemical and Biophysical Research Communications, vol.191, issue.3, 1993.
DOI : 10.1006/bbrc.1993.1349

J. R. Lakowicz and A. Balter, Analysis of excited-state processes by phase-modulation fluorescence spectroscopy, Biophysical Chemistry, vol.16, issue.2, pp.117-132, 1982.
DOI : 10.1016/0301-4622(82)85013-8

L. D. Landau and E. M. Lifshitz, Electrodynamics of Continuous Media, Course of Theoritical Physics, 1960.

A. Tatibouët, M. Demeunynck, C. Andraud, A. Collet, and J. Lhomme, Synthesis and study of an acridine stubstituted Tröger's base : preferential bindiing of the (-)isomer to B-DNA, Chem. Commun, pp.161-162, 1999.

J. J. Maki, M. Kauranen, and A. Persoons, Surface second-harmonic generation from chiral materials, Physical Review B, vol.51, issue.3, pp.1425-1434, 1995.
DOI : 10.1103/PhysRevB.51.1425

J. J. Maki, T. Verbiest, and M. Kauranen, Comparison of linearly and circularly polarized probes of second???order optical activity of chiral surfaces, The Journal of Chemical Physics, vol.105, issue.2, p.767, 1996.
DOI : 10.1063/1.471886

L. Moreaux, O. Sandre, M. Blanchard-desce, and J. Mertz, Membrane imaging by simultaneous second-harmonic generation and two-photon microscopy, Optics Letters, vol.25, issue.5, pp.320-322, 2000.
DOI : 10.1364/OL.25.000320

G. L. Aistrup, D. B. Sattelle, B. R. Martin, and E. D. Levin, Effects of toxic extracts and purified borbotoxins from Prorocentrum borbonicum (Dinophyceae) on vertebrate neuromuscular junctions, Toxicol Sci, vol.57, issue.2, pp.193-202, 2000.

M. Oheim, E. Beaurepaire, E. Chaigneau, J. Mertz, and S. Charpak, Two-photon microscopy in brain tissue: parameters influencing the imaging depth, Journal of Neuroscience Methods, vol.111, issue.1, pp.29-37, 2001.
DOI : 10.1016/S0165-0270(01)00438-1

N. Pagès, M. P. Sauviat, S. Bouvet, and F. Et-goudey-perrière, Toxicit?? du lindane sur la reproduction, Journal de la Soci??t?? de Biologie, vol.196, issue.4, pp.325-339, 2002.
DOI : 10.1051/jbio/2002196040325

J. P. Pérez, Optique géométrique et ondulatoire, 4iéme édition, 1994.

T. Poynard, P. Bedossa, P. Opolon, O. For-the, M. et al., Natural history of liver fibrosis progression in patients with chronic hepatitis C, The Lancet, vol.349, issue.9055, pp.825-857, 1997.
DOI : 10.1016/S0140-6736(96)07642-8

M. Guyot and J. Molgo, Effects of toxic extracts and purified borbotoxins from Prorocentrum borbonicum (Dinophyceae) on vertebrate neuromuscular junctions, Toxicon. Feb, vol.40, issue.2, pp.137-185, 2002.
URL : https://hal.archives-ouvertes.fr/hal-00193867

A. Rodger and B. Norden, Circular dichroism and linear dichroism, 1997.

L. B. Sandberg, W. Gray, and C. Franzblau, Elastin and elastic tissues, Advances in Experimental Medicine and Biology, 1977.
DOI : 10.1007/978-1-4684-9093-0

M. P. Sauviat, A. Colas, and N. Et-pages, Does lindane (gammahexachlorocyclohexane ) increase the rapid delayed rectifier outward K+ current (IKr) in frog atrial myocytes, BioMed Central Pharmacology, vol.2, p.15, 2002.
URL : https://hal.archives-ouvertes.fr/inserm-00123600

M. C. Klein, F. Hache, A. Roy, C. Flytzanis, and C. Payrastre, Off resonance second order optical activity of isotropic layers of chiral molecules: Observation of electric and magnetic contributions, The Journal of Chemical Physics, vol.108, issue.22, pp.9436-9443, 1998.
DOI : 10.1063/1.476394

C. Andraud, Strong chiroptical effects in surface second harmonic generation obtained for molecules exhibiting excitonic coupling chirality, Chem. Phys. Lett, vol.362, issue.103, 2002.
URL : https://hal.archives-ouvertes.fr/hal-00836943

S. Sheppard, C. J. Gu, and M. , Image-formation in 2-photon fluorescence microscopy, Optik, vol.86, pp.104-106, 1990.

J. M. Squirrell, D. L. Wokosin, J. G. White, and B. D. Bavister, Long-term twophoton fluorescence imaging of mammalian embryos without compromising viability, Nature Biotechnology, vol.17, issue.8, pp.763-770, 1999.
DOI : 10.1038/11698

B. C. Starcher and M. J. Galione, Purification and comparison of elastins from different animal species, Analytical Biochemistry, vol.74, issue.2, p.441, 1976.
DOI : 10.1016/0003-2697(76)90224-4

R. Stolle, M. Loddoch, and G. Marowsky, Theory of second harmonic circular dichroism at surfaces, Non linear Optics 8, p.79, 1994.

R. E. Thompson, D. R. Larson, and W. W. Webb, Precise Nanometer Localization Analysis for Individual Fluorescent Probes, Biophysical Journal, vol.82, issue.5, pp.2775-2783, 2002.
DOI : 10.1016/S0006-3495(02)75618-X

J. Trinick and L. Tskhovrebova, Titin: a molecular control freak, Trends in cell biology, pp.377-380, 1999.

T. Verbiest, M. Kauranen, J. J. Maki, M. N. Teerenstra, A. J. Schouten et al., Linearly polarized probes of surface chirality, The Journal of Chemical Physics, vol.103, issue.18, p.8296, 1995.
DOI : 10.1063/1.470714

G. W. Ware, US-EPA Office of drinking water health advisories, 1988.

W. Denk, J. Strickler, and W. W. Webb, Two-photon laser scanning fluorescence microscopy, Science, vol.248, issue.4951, pp.73-76, 1990.
DOI : 10.1126/science.2321027

R. D. Spencer and G. Weber, MEASUREMENTS OF SUBNANOSECOND FLUORESCENCE LIFETIMES WITH A CROSS-CORRELATION PHASE FLUOROMETER, Annals of the New York Academy of Sciences, vol.55, issue.1 Electronic As, pp.361-376, 1969.
DOI : 10.1007/BF01331053

C. Xu and W. W. Webb, Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm, Journal of the Optical Society of America B, vol.13, issue.3, pp.481-491, 1996.
DOI : 10.1364/JOSAB.13.000481

C. Xu, W. Zipfel, J. B. Shear, R. M. Williams, and W. W. Webb, Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy., Proceedings of the National Academy of Sciences, vol.93, issue.20, 1996.
DOI : 10.1073/pnas.93.20.10763

W. Yu, P. So, T. French, and E. Gratton, Fluorescence generalized polarization of cell membranes: a two-photon scanning microscopy approach, Biophysical Journal, vol.70, issue.2, pp.626-662, 1996.
DOI : 10.1016/S0006-3495(96)79646-7

R. Yuste and W. Denk, Dendritic spines as basic functional units of meuronal integration, Narture, vol.375, pp.682-684, 1995.

W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman et al., Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation, Proc. Natl. Acad. Sci. USA, pp.7075-7080, 2003.
DOI : 10.1073/pnas.0832308100