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Journal articles
Protection-based assays to measure aminoacyl-tRNA binding to translation initiation factors.
Abstract : To decipher the mechanisms of translation initiation, the stability of the complexes between tRNA and initiation factors has to be evaluated in a routine manner. A convenient method to measure the parameters of binding of an aminoacyl-tRNA to an initiation factor results from the property that, when specifically complexed to a protein, the aminoacyl-tRNA often resists spontaneous deacylation. This chapter describes the preparation of suitable aminoacyl-tRNA ligands and their use in evaluating the stability of their complexes with various initiation factors, such as e/aIF2 and e/aIF5B. The advantages and the limitations of the method are discussed.
https://hal-polytechnique.archives-ouvertes.fr/hal-00502061
Contributor : Yves Mechulam Connect in order to contact the contributor
Submitted on : Tuesday, July 13, 2010 - 10:31:54 AM
Last modification on : Tuesday, December 8, 2020 - 9:57:52 AM
Contributor : Yves Mechulam Connect in order to contact the contributor
Submitted on : Tuesday, July 13, 2010 - 10:31:54 AM
Last modification on : Tuesday, December 8, 2020 - 9:57:52 AM