Skip to Main content Skip to Navigation
Journal articles

Protection-based assays to measure aminoacyl-tRNA binding to translation initiation factors.

Yves Mechulam 1 Laurent Guillon 1 Laure Yatime 1 Sylvain Blanquet 1 Emmanuelle Schmitt 1, *
* Corresponding author
1 BIOC - Laboratoire de Biochimie de l'Ecole polytechnique
Abstract : To decipher the mechanisms of translation initiation, the stability of the complexes between tRNA and initiation factors has to be evaluated in a routine manner. A convenient method to measure the parameters of binding of an aminoacyl-tRNA to an initiation factor results from the property that, when specifically complexed to a protein, the aminoacyl-tRNA often resists spontaneous deacylation. This chapter describes the preparation of suitable aminoacyl-tRNA ligands and their use in evaluating the stability of their complexes with various initiation factors, such as e/aIF2 and e/aIF5B. The advantages and the limitations of the method are discussed.
Complete list of metadatas
https://hal-polytechnique.archives-ouvertes.fr/hal-00502061
Contributor : Yves Mechulam <>
Submitted on : Tuesday, July 13, 2010 - 10:31:54 AM
Last modification on : Thursday, March 5, 2020 - 6:20:14 PM

Identifiers

Collections

CNRS | X | X-BIOCH | X-DEP-BIO

Citation

Yves Mechulam, Laurent Guillon, Laure Yatime, Sylvain Blanquet, Emmanuelle Schmitt. Protection-based assays to measure aminoacyl-tRNA binding to translation initiation factors.. Methods in Enzymology, Elsevier, 2007, 430, pp.265-81. ⟨10.1016/S0076-6879(07)30011-6⟩. ⟨hal-00502061⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

166

Contact

Informations

CCSD