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Journal articles
Structural basis of RNA-dependent recruitment of glutamine to the genetic code.
Hiroyuki Oshikane
1
Kelly Sheppard
2
Shuya Fukai
1
Yuko Nakamura
1
Ryuichiro Ishitani
1
Tomoyuki Numata
1
R Lynn Sherrer
2
Liang Feng
2
Emmanuelle Schmitt
3
Michel Panvert
3
Sylvain Blanquet
3
Yves Mechulam
3
Dieter Söll
2
Osamu Nureki
1
Emmanuelle Schmitt 3
Author
Michel Panvert 3
Author
Sylvain Blanquet 3
Author
Yves Mechulam 3
Author
Dieter Söll 2
Author
Osamu Nureki 1
Author
1
DBI - Department of biological information-Tokyo Institute of Technology (Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa 226-8501, Japan. - Japan)
- TITECH - Tokyo Institute of Technology [Tokyo] (2-12-1 Ookayama, Meguro-ku, Tokyo, 152-8550 - Japan)
2
DMBB - Department of Molecular Biophysics and Biochemistry-Yale (Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA. - United States)
- Yale University [New Haven] (157 Church Street, New Haven, CT 06510-2100 - United States)
3
BIOC - Laboratoire de Biochimie de l'Ecole polytechnique (Ecole Polytechnique 91128 Palaiseau - France)
- X - École polytechnique (École polytechnique, 91128 Palaiseau Cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7654 (France)
Abstract : Glutaminyl-transfer RNA (Gln-tRNA(Gln)) in archaea is synthesized in a pretranslational amidation of misacylated Glu-tRNA(Gln) by the heterodimeric Glu-tRNA(Gln) amidotransferase GatDE. Here we report the crystal structure of the Methanothermobacter thermautotrophicus GatDE complexed to tRNA(Gln) at 3.15 angstroms resolution. Biochemical analysis of GatDE and of tRNA(Gln) mutants characterized the catalytic centers for the enzyme's three reactions (glutaminase, kinase, and amidotransferase activity). A 40 angstrom-long channel for ammonia transport connects the active sites in GatD and GatE. tRNA(Gln) recognition by indirect readout based on shape complementarity of the D loop suggests an early anticodon-independent RNA-based mechanism for adding glutamine to the genetic code.
Document type :
Journal articles
Complete list of metadatas
https://hal-polytechnique.archives-ouvertes.fr/hal-00502072
Contributor : Yves Mechulam <>
Submitted on : Tuesday, July 13, 2010 - 10:39:57 AM
Last modification on : Wednesday, July 29, 2020 - 4:10:05 PM
Contributor : Yves Mechulam <>
Submitted on : Tuesday, July 13, 2010 - 10:39:57 AM
Last modification on : Wednesday, July 29, 2020 - 4:10:05 PM
Identifiers
- HAL Id : hal-00502072, version 1
- DOI : 10.1126/science.1128470
- PUBMED : 16809540
Citation
Hiroyuki Oshikane, Kelly Sheppard, Shuya Fukai, Yuko Nakamura, Ryuichiro Ishitani, et al.. Structural basis of RNA-dependent recruitment of glutamine to the genetic code.. Science, American Association for the Advancement of Science, 2006, 312 (5782), pp.1950-4. ⟨10.1126/science.1128470⟩. ⟨hal-00502072⟩
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