Deep and fast live imaging with two-photon scanned light-sheet microscopy

Abstract : We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology. Cop. 2011 Nature America, Inc. All rights reserved.
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T.V. Truong, Willy Supatto, D.S. Koos, J.M. Choi, S.E. Fraser. Deep and fast live imaging with two-photon scanned light-sheet microscopy. Nature Methods, Nature Publishing Group, 2011, 8 (9), pp.757. ⟨10.1038/nmeth.1652⟩. ⟨hal-00803780⟩

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