Deep and fast live imaging with two-photon scanned light-sheet microscopy

T.V. Truong (1) , Willy Supatto (1, 2) , D.S. Koos (1) , J.M. Choi (1) , S.E. Fraser (1)

1 Beckman Institute
2 LOB - Laboratoire d'optique et biosciences

We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology. Cop. 2011 Nature America, Inc. All rights reserved.

T.V. Truong, Willy Supatto, D.S. Koos, J.M. Choi, S.E. Fraser. Deep and fast live imaging with two-photon scanned light-sheet microscopy. Nature Methods, 2011, 8 (9), pp.757. ⟨10.1038/nmeth.1652⟩. ⟨hal-00803780⟩