Abstract : We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology. Cop. 2011 Nature America, Inc. All rights reserved.
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Journal articles
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https://hal-polytechnique.archives-ouvertes.fr/hal-00803780
Contributor : Denis Roura
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Submitted on : Monday, March 25, 2013 - 11:20:20 AM
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