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Spectroscopic analysis of keratin endogenous signal for skin multiphoton microscopy
Abstract : We recorded one-photon excited fluorescence (1PEF) and two-photon excited fluorescence (2PEF) spectra of purified keratin from human epidermis, and determined the action cross section of this endogenous chromophore. We used this spectroscopic analysis to analyse multiphoton images of skin biopsies and assign the intrinsic fluorescence signals in the epidermis. We observed a good agreement between in situ and in vitro 2PEF spectra of keratin. This study provides a comprehensive characterization of the 2PEF signal of the keratins from the epidermis, and will be of practical interest for multiphoton imaging of the skin. © 2005 Optical Society of America
https://hal-polytechnique.archives-ouvertes.fr/hal-00807874
Contributor : Denis Roura Connect in order to contact the contributor
Submitted on : Thursday, May 15, 2014 - 1:19:02 PM
Last modification on : Thursday, July 22, 2021 - 9:12:02 AM
Long-term archiving on: : Friday, August 15, 2014 - 10:35:53 AM
Contributor : Denis Roura Connect in order to contact the contributor
Submitted on : Thursday, May 15, 2014 - 1:19:02 PM
Last modification on : Thursday, July 22, 2021 - 9:12:02 AM
Long-term archiving on: : Friday, August 15, 2014 - 10:35:53 AM
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