Amplification and temporal filtering during gradient sensing by nerve growth cones probed with a microfluidic assay - Archive ouverte HAL Access content directly
Journal Articles Biophysical Journal Year : 2012

Amplification and temporal filtering during gradient sensing by nerve growth cones probed with a microfluidic assay

(1) , (1, 2) , (1) , (1, 3) , (4) , (3) , (1)
1
2
3
4

Abstract

Nerve growth cones (GCs) are chemical sensors that convert graded extracellular cues into oriented axonal motion. To ensure a sensitive and robust response to directional signals in complex and dynamic chemical landscapes, GCs are presumably able to amplify and filter external information. How these processing tasks are performed remains however poorly known. Here, we probe the signal-processing capabilities of single GCs during ?-Aminobutyric acid (GABA) directional sensing with a shear-free microfluidic assay that enables systematic measurements of the GC output response to variable input gradients. By measuring at the single molecule level the polarization of GABA A chemoreceptors at the GC membrane, as a function of the external GABA gradient, we find that GCs act as i), signal amplifiers over a narrow range of concentrations, and ii), low-pass temporal filters with a cutoff frequency independent of stimuli conditions. With computational modeling, we determine that these systems-level properties arise at a molecular level from the saturable occupancy response and the lateral dynamics of GABA A receptors. Cop. 2012 Biophysical Society.
Fichier principal
Vignette du fichier
S0006349512009666.pdf (873.2 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-00817156 , version 1 (23-05-2014)

Identifiers

Cite

Mathieu Morel, Vasyl Shynkar, Jean-Christophe Galas, Isabelle Dupin, Cédric Bouzigues, et al.. Amplification and temporal filtering during gradient sensing by nerve growth cones probed with a microfluidic assay. Biophysical Journal, 2012, 103 (8), pp.1648-1656. ⟨10.1016/j.bpj.2012.08.040⟩. ⟨hal-00817156⟩
629 View
539 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More