Amplification and temporal filtering during gradient sensing by nerve growth cones probed with a microfluidic assay

Mathieu Morel 1 Vasyl Shynkar 1, 2 Jean-Christophe Galas 1 Isabelle Dupin 1, 3 Cédric Bouzigues 4 Vincent Studer 3 Maxime Dahan 1, *
* Corresponding author
1 LKB (Jussieu) - Laboratoire Kastler Brossel
2 Fondation Pierre-Gilles de Gennes
3 Interdisciplinary Institute for Neuroscience
4 LOB - Laboratoire d'optique et biosciences
Abstract : Nerve growth cones (GCs) are chemical sensors that convert graded extracellular cues into oriented axonal motion. To ensure a sensitive and robust response to directional signals in complex and dynamic chemical landscapes, GCs are presumably able to amplify and filter external information. How these processing tasks are performed remains however poorly known. Here, we probe the signal-processing capabilities of single GCs during ?-Aminobutyric acid (GABA) directional sensing with a shear-free microfluidic assay that enables systematic measurements of the GC output response to variable input gradients. By measuring at the single molecule level the polarization of GABA A chemoreceptors at the GC membrane, as a function of the external GABA gradient, we find that GCs act as i), signal amplifiers over a narrow range of concentrations, and ii), low-pass temporal filters with a cutoff frequency independent of stimuli conditions. With computational modeling, we determine that these systems-level properties arise at a molecular level from the saturable occupancy response and the lateral dynamics of GABA A receptors. Cop. 2012 Biophysical Society.
Document type :
Journal articles
Complete list of metadatas

Cited literature [40 references]  Display  Hide  Download
https://hal-polytechnique.archives-ouvertes.fr/hal-00817156
Contributor : Denis Roura <>
Submitted on : Friday, May 23, 2014 - 3:19:37 PM
Last modification on : Thursday, March 21, 2019 - 2:53:53 PM
Long-term archiving on : Monday, April 3, 2017 - 9:00:17 AM

File

S0006349512009666.pdf
Publisher files allowed on an open archive

Identifiers

Collections

X-LOB | ALLINSP | X | PARISTECH | UPMC | LKB | ENS-PARIS | PSL | X-DEP-BIO | SORBONNE-UNIVERSITE | SU-SCIENCES

Citation

Mathieu Morel, Vasyl Shynkar, Jean-Christophe Galas, Isabelle Dupin, Cédric Bouzigues, et al.. Amplification and temporal filtering during gradient sensing by nerve growth cones probed with a microfluidic assay. Biophysical Journal, Biophysical Society, 2012, 103 (8), pp.1648-1656. ⟨10.1016/j.bpj.2012.08.040⟩. ⟨hal-00817156⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

1079

Files downloads

417

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD