Subpicosecond UV spectroscopy of carbonmonoxy-myoglobin: Absorption and circular dichroism studies

Thibault Dartigalongue 1, 2 Claire Niezborala 1 François Hache 1
1 LOB - Laboratoire d'optique et biosciences
2 Department of Physics [Toronto]
Abstract : A thorough absorption and circular dichroism study is performed in carbonmonoxy-myoglobin with a sub-picosecond visible pump, ultraviolet probe experiment. Differential absorption in the 220-360 nm range shows that the time-resolved response mainly comes from the heme and that aromatic amino acids do not contribute significantly. Time-resolved CD at 260 nm shows no dynamics and confirms this result. On the contrary, a strong CD dynamics is observed at 230 nm. This signal could originate from transient deformation of the a-helices in the protein. Cop. the Owner Societies.
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Submitted on : Wednesday, May 22, 2013 - 1:36:56 PM
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Thibault Dartigalongue, Claire Niezborala, François Hache. Subpicosecond UV spectroscopy of carbonmonoxy-myoglobin: Absorption and circular dichroism studies. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2007, 9 (13), pp.1611. ⟨10.1039/b616173a⟩. ⟨hal-00824060⟩

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