Chiroptical effects in the second harmonic signal of collagens I and IV

Abstract : We performed polarization-resolved surface second harmonic generation (SHG) experiments on thin films of collagen I and IV molecules, as well as conventional CD measurements. We found that collagen IV presents little CD and no SHG optical activity, whereas collagen I exhibits large chiroptical effects involving both one-electron and excitonic coupling mechanisms. We estimated that these chiral components enhance the SHG signal from fibrillar collagen in biological tissues by typically a factor of 2. By comparing the distinct behaviors of collagens I and IV in SHG microscopy and in surface SHG experiments, we concluded that SHG microscopy is a sensitive probe of the micrometer-scale structural organization of collagen in biological tissues. Cop. 2005 American Chemical Society.
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Ana-Maria Pena, Thierry Boulesteix, Thibault Dartigalongue, Marie-Claire Schanne-Klein. Chiroptical effects in the second harmonic signal of collagens I and IV. Journal of the American Chemical Society, American Chemical Society, 2005, 127 (29), pp.10314. ⟨10.1021/ja0520969⟩. ⟨hal-00829225⟩

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