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Enhanced release of nitric oxide from endothelial cells under anoxia

A. F. Vanin 1 Anny Slama-Schwok 2 L. M. Bevers 3 E. van Faassen 4
1 N. N. Semenov Institute of Chemical Physics
2 LOB - Laboratoire d'optique et biosciences
3 Nephrology and Hypertension
4 Faculty of Sciences
Abstract : Endothelial nitric oxide synthase (eNOS) is capable of releasing free NO from nitrite in vitro under anoxiaa. Therefore the anoxic nitrite reductase pathway might provide a significant alternative source of NO for tissues under acute hypoxia. This hypothesis was tested by NO trapping in cultured endothelial (bEND.3) cells using iron-dithiocarbamate traps and EPR. Yields (in pmol) from NO trapping in 7.5*106 endothelial cells for 20 min at 37°C. Oxia is in an atmosphere with 5% CO 2 and 20% O 2 , anoxia is under argon. The supplements were added to medium.
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https://hal-polytechnique.archives-ouvertes.fr/hal-00844503
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Submitted on : Monday, July 15, 2013 - 4:14:38 PM
Last modification on : Tuesday, July 14, 2020 - 11:04:05 AM

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A. F. Vanin, Anny Slama-Schwok, L. M. Bevers, E. van Faassen. Enhanced release of nitric oxide from endothelial cells under anoxia. Annual Meeting of the German Society for Microcirculation and Vascular Biology, Oct 2006, Munich, Germany. pp.548. ⟨hal-00844503⟩

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