Enhanced release of nitric oxide from endothelial cells under anoxia

A. F. Vanin; Anny Slama-Schwok; L. M. Bevers; E. van Faassen

Enhanced release of nitric oxide from endothelial cells under anoxia

A. F. Vanin ¹ Anny Slama-Schwok ² L. M. Bevers ³ E. van Faassen ⁴

1 N. N. Semenov Institute of Chemical Physics

2 LOB - Laboratoire d'optique et biosciences

3 Nephrology and Hypertension

4 Faculty of Sciences

Abstract : Endothelial nitric oxide synthase (eNOS) is capable of releasing free NO from nitrite in vitro under anoxiaa. Therefore the anoxic nitrite reductase pathway might provide a significant alternative source of NO for tissues under acute hypoxia. This hypothesis was tested by NO trapping in cultured endothelial (bEND.3) cells using iron-dithiocarbamate traps and EPR. Yields (in pmol) from NO trapping in 7.5*106 endothelial cells for 20 min at 37°C. Oxia is in an atmosphere with 5% CO 2 and 20% O 2 , anoxia is under argon. The supplements were added to medium.

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Submitted on : Monday, July 15, 2013 - 4:14:38 PM
Last modification on : Wednesday, January 23, 2019 - 10:29:00 AM

Enhanced release of nitric oxide from endothelial cells under anoxia

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Enhanced release of nitric oxide from endothelial cells under anoxia

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