A selective and sensitive method for quantitation of lysergic acid diethylamide (LSD) in whole blood by gas chromatography-ion trap tandem mass spectrometry.

Danielle Libong 1 Stéphane Bouchonnet 1 Ivan Ricordel 2
1 DCMR - Laboratoire des mécanismes réactionnels
2 Laboratoire de Toxicologie de la Préfecture de Police
Abstract : A gas chromatography-ion trap tandem mass spectrometry (GC-ion trap MS-MS) method for detection and quantitation of LSD in whole blood is presented. The sample preparation process, including a solid-phase extraction step with Bond Elut cartridges, was performed with 2 mL of whole blood. Eight microliters of the purified extract was injected with a cold on-column injection method. Positive chemical ionization was performed using acetonitrile as reagent gas; LSD was detected in the MS-MS mode. The chromatograms obtained from blood extracts showed the great selectivity of the method. GC-MS quantitation was performed using lysergic acid methylpropylamide as the internal standard. The response of the MS was linear for concentrations ranging from 0.02 ng/mL (detection threshold) to 10.0 ng/mL. Several parameters such as the choice of the capillary column, the choice of the internal standard and that of the ionization mode (positive CI vs. EI) were rationalized. Decomposition pathways under both ionization modes were studied. Within-day and between-day stability were evaluated.
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Journal articles
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https://hal-polytechnique.archives-ouvertes.fr/hal-00917367
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Submitted on : Thursday, December 12, 2013 - 8:16:14 PM
Last modification on : Wednesday, March 20, 2019 - 12:12:02 PM

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  • HAL Id : hal-00917367, version 1
  • PUBMED : 12587679

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Danielle Libong, Stéphane Bouchonnet, Ivan Ricordel. A selective and sensitive method for quantitation of lysergic acid diethylamide (LSD) in whole blood by gas chromatography-ion trap tandem mass spectrometry.. Journal of Analytical Toxicology, Oxford University Press (OUP): Policy F, 2003, 27 (1), pp.24-29. ⟨hal-00917367⟩

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