Interactions between vascular endothelial cells and nanoparticles investigated by single-cell mechanics

Christopher C. Zimmer 1 Ying X. Liu 1 Gang-Yu Liu 1 Joshua T. Morgan 2 Ian M. Kennedy 2 Abdul I. Barakat 2, 3
1 Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
2 Univ Calif Davis, Dept Mech & Aeronaut Engn, Davis, CA 95616 USA
3 LadHyX - Laboratoire d'hydrodynamique
Abstract : Inhalation of airborne nanoparticles (NPs) has been correlated with cardiopulmonary disease. To better understand the underlying mechanisms, human aortic endothelial cells (HAECs) were exposed in vitro to zinc oxide (ZnO) and silicon dioxide (SiO2) NPs at various doses. By using atomic force microscopy based single-cell compression, we observed that ZnO NPs at 10 µg/ml produced stiffening of the cellular membrane and cytoskeleton. At higher ZnO NP doses, cell volume increased by up to 280%. SiO2 NPs had a similar but milder effect. A simple balloon model based on Hertzian mechanics yielded a membrane Young's modulus (Em) increase from 5.0 ± 2.1 MPa to 7.7 ± 1.8 MPa for ZnO NPs and to only 5.49 ± 0.75 MPa for SiO2 NPs. The results can be rationalized by unregulated ion influx leading to increased intracellular osmotic pressure. Single-cell mechanics provides sensitive and quantitative measurements that yield insight into NP - cell interactions.
Type de document :
Communication dans un congrès
243rd ACS National Meeting & Exposition, Mar 2012, San Diego, United States. American Chemical Society, 243, pp.656, 2012
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https://hal-polytechnique.archives-ouvertes.fr/hal-01025968
Contributeur : Denis Roura <>
Soumis le : mardi 22 juillet 2014 - 15:19:33
Dernière modification le : jeudi 10 mai 2018 - 02:02:05

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Christopher C. Zimmer, Ying X. Liu, Gang-Yu Liu, Joshua T. Morgan, Ian M. Kennedy, et al.. Interactions between vascular endothelial cells and nanoparticles investigated by single-cell mechanics. 243rd ACS National Meeting & Exposition, Mar 2012, San Diego, United States. American Chemical Society, 243, pp.656, 2012. 〈hal-01025968〉

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