Dynamic monitoring of cell mechanical properties using profile microindentation.

Abstract : We have developed a simple and relatively inexpensive system to visualize adherent cells in profile while measuring their mechanical properties using microindentation. The setup allows simultaneous control of cell microenvironment by introducing a micropipette for the delivery of soluble factors or other cell types. We validate this technique against atomic force microscopy measurements and, as a proof of concept, measure the viscoelastic properties of vascular endothelial cells in terms of an apparent stiffness and a dimensionless parameter that describes stress relaxation. Furthermore, we use this technique to monitor the time evolution of these mechanical properties as the cells' actin is depolymerized using cytochalasin-D.
Type de document :
Article dans une revue
Scientific Reports, Nature Publishing Group, 2015, 6, pp.21529. 〈10.1038/srep21529〉
Liste complète des métadonnées

Littérature citée [40 références]  Voir  Masquer  Télécharger

https://hal-polytechnique.archives-ouvertes.fr/hal-01299614
Contributeur : Denis Roura <>
Soumis le : jeudi 7 avril 2016 - 21:58:38
Dernière modification le : jeudi 10 mai 2018 - 02:02:41

Fichier

srep21529.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Citation

Lionel Guillou, A Babataheri, P-H Puech, A I Barakat, J Husson. Dynamic monitoring of cell mechanical properties using profile microindentation.. Scientific Reports, Nature Publishing Group, 2015, 6, pp.21529. 〈10.1038/srep21529〉. 〈hal-01299614〉

Partager

Métriques

Consultations de la notice

328

Téléchargements de fichiers

177