Dynamic monitoring of cell mechanical properties using profile microindentation.

Abstract : We have developed a simple and relatively inexpensive system to visualize adherent cells in profile while measuring their mechanical properties using microindentation. The setup allows simultaneous control of cell microenvironment by introducing a micropipette for the delivery of soluble factors or other cell types. We validate this technique against atomic force microscopy measurements and, as a proof of concept, measure the viscoelastic properties of vascular endothelial cells in terms of an apparent stiffness and a dimensionless parameter that describes stress relaxation. Furthermore, we use this technique to monitor the time evolution of these mechanical properties as the cells' actin is depolymerized using cytochalasin-D.
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Lionel Guillou, A Babataheri, P-H Puech, a I Barakat, J Husson. Dynamic monitoring of cell mechanical properties using profile microindentation.. Scientific Reports, Nature Publishing Group, 2015, 6, pp.21529. ⟨10.1038/srep21529⟩. ⟨hal-01299614⟩

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